Literature DB >> 8017952

Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.

S Wittmann1, F Shareck, D Kluepfel, R Morosoli.   

Abstract

The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.

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Year:  1994        PMID: 8017952      PMCID: PMC201545          DOI: 10.1128/aem.60.5.1701-1703.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  13 in total

1.  Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase).

Authors:  T Ooi; A Shinmyo; H Okada; S Murao; T Kawaguchi; M Arai
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

2.  Sequences of three genes specifying xylanases in Streptomyces lividans.

Authors:  F Shareck; C Roy; M Yaguchi; R Morosoli; D Kluepfel
Journal:  Gene       Date:  1991-10-30       Impact factor: 3.688

Review 3.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

4.  Cloning of a second xylanase-encoding gene of Streptomyces lividans 66.

Authors:  S Vats-Mehta; P Bouvrette; F Shareck; R Morosoli; D Kluepfel
Journal:  Gene       Date:  1990-01-31       Impact factor: 3.688

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca.

Authors:  G Lao; G S Ghangas; E D Jung; D B Wilson
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

7.  Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme.

Authors:  N Arcand; D Kluepfel; F W Paradis; R Morosoli; F Shareck
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

8.  Identification of a celE-binding protein and its potential role in induction of the celE gene in Thermomonospora fusca.

Authors:  E S Lin; D B Wilson
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

9.  Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain.

Authors:  R Nakai; S Horinouchi; T Beppu
Journal:  Gene       Date:  1988-05-30       Impact factor: 3.688

10.  Cloning of the xylanase gene of Streptomyces lividans.

Authors:  F Mondou; F Shareck; R Morosoli; D Kluepfel
Journal:  Gene       Date:  1986       Impact factor: 3.688

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  11 in total

1.  An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose.

Authors:  M W Bauer; L E Driskill; W Callen; M A Snead; E J Mathur; R M Kelly
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

2.  Substrate-binding domains of glycanases from Streptomyces lividans: characterization of a new family of xylan-binding domains.

Authors:  C Dupont; M Roberge; F Shareck; R Morosoli; D Kluepfel
Journal:  Biochem J       Date:  1998-02-15       Impact factor: 3.857

3.  Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8.

Authors:  A L Garda; J M Fernández-Abalos; P Sánchez; A Ruiz-Arribas; R I Santamaría
Journal:  Biochem J       Date:  1997-06-01       Impact factor: 3.857

4.  The synthesis of the Streptomyces reticuli cellulase (avicelase) is regulated by both activation and repression mechanisms.

Authors:  S Walter; H Schrempf
Journal:  Mol Gen Genet       Date:  1996-05-23

5.  Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA).

Authors:  C Manin; F Shareek; R Morosoli; D Kluepfel
Journal:  Biochem J       Date:  1994-09-01       Impact factor: 3.857

6.  Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB.

Authors:  D L Zechel; S He; C Dupont; S G Withers
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857

7.  Studies of Streptomyces reticuli cel-1 (cellulase) gene expression in Streptomyces strains, Escherichia coli, and Bacillus subtilis.

Authors:  S Walter; H Schrempf
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

8.  Effect of signal peptide alterations and replacement on export of xylanase A in Streptomyces lividans.

Authors:  N Pagé; D Kluepfel; F Shareck; R Morosoli
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

9.  Kinetics and thermodynamics of a novel endoglucanase (CMCase) from Gymnoascella citrina produced under solid-state condition.

Authors:  Abdul Jabbar; Muhammad Hamid Rashid; Muhammad Rizwan Javed; Raheela Perveen; Muhammad Aslam Malana
Journal:  J Ind Microbiol Biotechnol       Date:  2008-01-29       Impact factor: 3.346

10.  Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost.

Authors:  Antonella Amore; Olimpia Pepe; Valeria Ventorino; Leila Birolo; Chiara Giangrande; Vincenza Faraco
Journal:  Microb Cell Fact       Date:  2012-12-26       Impact factor: 5.328

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