Literature DB >> 8015368

A rapid method for the preparation of ganglioside Glac2 (GD3).

R Jennemann1, H Wiegandt.   

Abstract

A method is described for the preparation of ganglioside Glac2 [(II3(NeuAc)2-LacCer, GD3] from cream of bovine milk using liquid-phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14-15 L of bovine milk, yields approximately 70 mg of pure ganglioside Glac2. The sialic acid constituent of ganglioside Glac2 isolated from bovine milk cream consists solely of the N-acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing-4-enine and the 22:0 (behenic acid) and 23:0 fatty acids.

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Year:  1994        PMID： 8015368     DOI： 10.1007/bf02537191

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  46 in total

1.  [FURTHER GANGLIOSIDES FROM THE HUMAN BRAIN].

Authors:  R KUHN; H WIEGANDT
Journal:  Z Naturforsch B       Date:  1964-03       Impact factor: 1.047

2.  Gangliosides of human, bovine and rabbit retina.

Authors:  M Holm; J E Månsson; M T Vanier; L Svennerholm
Journal:  Biochim Biophys Acta       Date:  1972-10-05

3.  A new procedure for the extraction, purification and fractionation of brain gangliosides.

Authors:  G Tettamanti; F Bonali; S Marchesini; V Zambotti
Journal:  Biochim Biophys Acta       Date:  1973-01-19

4.  Antibody response to immunization with ganglioside GD3 and GD3 congeners (lactones, amide and gangliosidol) in patients with malignant melanoma.

Authors:  G Ritter; E Boosfeld; R Adluri; M Calves; H F Oettgen; L J Old; P Livingston
Journal:  Int J Cancer       Date:  1991-05-30       Impact factor: 7.396

5.  Characterization of two molecular species GD3 ganglioside from bovine buttermilk.

Authors:  B Hauttecoeur; S Sonnino; R Ghidoni
Journal:  Biochim Biophys Acta       Date:  1985-02-08

6.  Characterization of a human melanoma-associated ganglioside antigen defined by a monoclonal antibody, 4.2.

Authors:  E Nudelman; S Hakomori; R Kannagi; S Levery; M Y Yeh; K E Hellström; I Hellström
Journal:  J Biol Chem       Date:  1982-11-10       Impact factor: 5.157

7.  Characterization of B and H blood-group active glycosphingolopids from human B erythrocyte membranes.

Authors:  P Hanfland
Journal:  Chem Phys Lipids       Date:  1975-11       Impact factor: 3.329

8.  Evidence for several cell populations in human thyroid with distinct glycosphingolipid patterns.

Authors:  B Bouchon; J Portoukalian; A M Madec; J Orgiazzi
Journal:  Biochim Biophys Acta       Date:  1990-01-23

9.  Glycosphingolipid composition of human semen.

Authors:  G Ritter; W Krause; R Geyer; S Stirm; H Wiegandt
Journal:  Arch Biochem Biophys       Date:  1987-09       Impact factor: 4.013

10.  Biochemical and serological characteristics of natural 9-O-acetyl GD3 from human melanoma and bovine buttermilk and chemically O-acetylated GD3.

Authors:  G Ritter; E Boosfeld; E Markstein; R K Yu; S L Ren; W B Stallcup; H F Oettgen; L J Old; P O Livingston
Journal:  Cancer Res       Date:  1990-03-01       Impact factor: 12.701
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  2 in total

1.  Elevation of GM2 ganglioside during ethanol-induced apoptotic neurodegeneration in the developing mouse brain.

Authors:  Mitsuo Saito; Goutam Chakraborty; Relish Shah; Rui-Fen Mao; Asok Kumar; Dun-Sheng Yang; Kostantin Dobrenis; Mariko Saito
Journal:  J Neurochem       Date:  2012-03-20       Impact factor: 5.372

2.  Apoptosis of human breast carcinoma cells in the presence of disialosyl gangliosides: II. Treatment of SKBR3 cells with GD3 and GD1b gangliosides.

Authors:  Rui Ma; Atanas Koulov; Christopher Moulton; Manju Basu; Sipra Banerjee; Holly Goodson; Subhash Basu
Journal:  Glycoconj J       Date:  2004       Impact factor: 3.009
  2 in total

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