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Literature DB >> 8001165

A luciferase expression system for Physarum that facilitates analysis of regulatory elements.

Abstract

We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.

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Year: 1994 PMID： 8001165 DOI： 10.1007/BF00313799

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

17 in total

1. Transient expression in Physarum of a chloramphenicol acetyltransferase gene under the control of actin gene promoters.

Authors: T G Burland; J Bailey; L Adam; M J Mukhopadhyay; W F Dove; D Pallotta
Journal: Curr Genet Date: 1992-04 Impact factor: 3.886

2. The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter.

Authors: G Pierron; D Pallotta; M Bénard
Journal: Mol Cell Biol Date: 1999-05 Impact factor: 4.272

2 in total

A luciferase expression system for Physarum that facilitates analysis of regulatory elements.

1. Transient expression in Physarum of a chloramphenicol acetyltransferase gene under the control of actin gene promoters.

2. Establishment of a transient expression system for Dictyostelium discoideum.

3. Fission yeast promoter-probe vectors based on hygromycin resistance.

4. Advantages of firefly luciferase as a reporter gene: application to the interleukin-2 gene promoter.

Review 5. The structure and function of yeast ARS elements.

6. In vitro assembly of microtubule proteins from myxamoebae of Physarum polycephalum.

7. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

8. A novel circadian phenotype based on firefly luciferase expression in transgenic plants.

9. Firefly luciferase gene: structure and expression in mammalian cells.

10. Expression of the three unlinked isocoding actin genes of Physarum polycephalum.

1. Homologous gene replacement in Physarum.

2. The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter.