Literature DB >> 7993100

Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.

T C Charles1, S L Doty, E W Nester.   

Abstract

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

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Year:  1994        PMID： 7993100      PMCID： PMC201960          DOI： 10.1128/aem.60.11.4192-4194.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

1.  Electroporation of megaplasmids into Agrobacterium.

Authors:  T Mozo; P J Hooykaas
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076

2.  Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein.

Authors:  G A Cangelosi; R G Ankenbauer; E W Nester
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

3.  Sugar-mediated induction of Agrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides.

Authors:  R G Ankenbauer; E W Nester
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

4.  A chromosomal Agrobacterium tumefaciens gene required for effective plant signal transduction.

Authors:  M L Huang; G A Cangelosi; W Halperin; E W Nester
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

5.  Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides.

Authors:  N Shimoda; A Toyoda-Yamamoto; J Nagamine; S Usami; M Katayama; Y Sakagami; Y Machida
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

6.  virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens.

Authors:  S E Stachel; P C Zambryski
Journal:  Cell       Date:  1986-08-01       Impact factor: 41.582

7.  Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58.

Authors:  T J Close; D Zaitlin; C I Kado
Journal:  Plasmid       Date:  1984-09       Impact factor: 3.466

8.  The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member.

Authors:  S L Doty; M Chang; E W Nester
Journal:  J Bacteriol       Date:  1993-12       Impact factor: 3.490

9.  Association of the virD2 protein with the 5' end of T strands in Agrobacterium tumefaciens.

Authors:  C Young; E W Nester
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

10.  A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.

Authors:  S E Stachel; G An; C Flores; E W Nester
Journal:  EMBO J       Date:  1985-04       Impact factor: 11.598
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  9 in total

1.  Combined genetic and physical map of the complex genome of Agrobacterium tumefaciens.

Authors:  B W Goodner; B P Markelz; M C Flanagan; C B Crowell; J L Racette; B A Schilling; L M Halfon; J S Mellors; G Grabowski
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Inability to catabolize galactose leads to increased ability to compete for nodule occupancy in Sinorhizobium meliloti.

Authors:  Barney A Geddes; Ivan J Oresnik
Journal:  J Bacteriol       Date:  2012-07-13       Impact factor: 3.490

3.  Site-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE).

Authors:  John R Heil; Jiujun Cheng; Trevor C Charles
Journal:  J Vis Exp       Date:  2012-03-16       Impact factor: 1.355

4.  The chvH locus of Agrobacterium encodes a homologue of an elongation factor involved in protein synthesis.

Authors:  W T Peng; L M Banta; T C Charles; E W Nester
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

5.  The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages.

Authors:  M T Alvarez-Martinez; J Machold; C Weise; H Schmidt-Eisenlohr; C Baron; B Rouot
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

6.  Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts.

Authors:  C Bélanger; I Loubens; E W Nester; P Dion
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490

7.  Strain engineering by genome mass transfer: efficient chromosomal trait transfer method utilizing donor genomic DNA and recipient recombineering hosts.

Authors:  James A Williams; Jeremy Luke; Clague Hodgson
Journal:  Mol Biotechnol       Date:  2009-05-20       Impact factor: 2.695

8.  Mutational analysis of the input domain of the VirA protein of Agrobacterium tumefaciens.

Authors:  S L Doty; M C Yu; J I Lundin; J D Heath; E W Nester
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

9.  A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid.

Authors:  Luoping Li; Yonghui Jia; Qingming Hou; Trevor C Charles; Eugene W Nester; Shen Q Pan
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-06       Impact factor: 11.205
  9 in total

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