Literature DB >> 7980388

Substrate and inhibitor specificities of the thermostable alcohol dehydrogenase allozymes ADH-71k and ADH-FCh.D. of Drosophila melanogaster.

K T Eisses1, S L Davies, G K Chambers.   

Abstract

Purified thermostable alcohol dehydrogenase allozymes ADH-71k and ADH-FCh.D. of Drosophila melanogaster have been compared with the two common enzyme forms ADH-F and ADH-S. Enzyme kinetic parameters for various primary and secondary alcohols were determined under standard conditions used previously. Both ADH-71k and ADH-FCh.D. show ADH-S-like reaction kinetics and Km values, due to retrograde evolution at site 214, Pro-->Ser. Inhibition studies with alcohol dehydrogenase inhibitors pyrazole, 4-methylpyrazole, and cibacron blue 3GA were also performed. Activity measurements on crude extracts of larvae and flies from isogenic lines of ADH-FCh.D. revealed a consistently higher activity than in ADH-71k-containing strains, in contrast to the original strains.

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Year:  1994        PMID： 7980388     DOI： 10.1007/BF00554418

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  41 in total

1.  A common ancestor for human placental 17 beta-hydroxysteroid dehydrogenase, Streptomyces coelicolor actIII protein, and Drosophila melanogaster alcohol dehydrogenase.

Authors:  M E Baker
Journal:  FASEB J       Date:  1990-02-01       Impact factor: 5.191

2.  The alcohol dehydrogenase alleloenzymes AdhS and AdhF from the fruitfly Drosophila melanogaster: an enzymatic rate assay to determine the active-site concentration.

Authors:  J O Winberg; R Hovik; J S McKinley-McKee
Journal:  Biochem Genet       Date:  1985-04       Impact factor: 1.890

3.  Structural analysis of an electrophoretically cryptic alcohol dehydrogenase variant from an Australian population of Drosophila melanogaster.

Authors:  G K Chambers; W G Laver; S Campbell; J B Gibson
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

4.  Analysis of the gene encoding the multifunctional alcohol dehydrogenase allozyme ADH-71k of Drosophila melanogaster.

Authors:  K T Eisses; A J Andriesse; A Douwe de Boer; G E Thörig; P J Weisbeek
Journal:  Mol Biol Evol       Date:  1990-09       Impact factor: 16.240

5.  Alcohol dehydrogenase controls the flux from ethanol into lipids in Drosophila larvae. A 13C NMR study.

Authors:  A Freriksen; D Seykens; W Scharloo; P W Heinstra
Journal:  J Biol Chem       Date:  1991-11-15       Impact factor: 5.157

6.  Evidence for a multiple function of the alcohol dehydrogenase allozyme ADH71k of Drosophila melanogaster.

Authors:  K T Eisses; W G Schoonen; W Scharloo; G E Thörig
Journal:  Comp Biochem Physiol B       Date:  1985

Review 7.  Kinetic interpretations of active site topologies and residue exchanges in Drosophila alcohol dehydrogenases.

Authors:  J O Winberg; J S McKinley-McKee
Journal:  Int J Biochem       Date:  1992-02

8.  Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone.

Authors:  C Benyajati; N Wang; A Reddy; E Weinberg; W Sofer
Journal:  Nucleic Acids Res       Date:  1980-12-11       Impact factor: 16.971

9.  Alcohol dehydrogenase from the fruitfly Drosophila melanogaster. Inhibition studies of the alleloenzymes AdhS and AdhUF.

Authors:  J O Winberg; D R Thatcher; J S McKinley-McKee
Journal:  Biochim Biophys Acta       Date:  1982-05-21

10.  An electrophoretically cryptic alcohol dehydrogenase variant in Drosophila melanogaster. I. Activity ratios, thermostability, genetic localization and comparison with two other thermostable variants.

Authors:  J B Gibson; G K Chambers; A V Wilks; J G Oakeshott
Journal:  Aust J Biol Sci       Date:  1980-08
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  1 in total

1.  Theoretical calculations of the catalytic triad in short-chain alcohol dehydrogenases/reductases.

Authors:  Osman A B S M Gani; Olayiwola A Adekoya; Laura Giurato; Francesca Spyrakis; Pietro Cozzini; Salvatore Guccione; Jan-Olof Winberg; Ingebrigt Sylte
Journal:  Biophys J       Date:  2007-11-02       Impact factor: 4.033
  1 in total

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