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Literature DB >> 7971278

Retroviral vector sequences inhibit human beta-globin gene expression in transgenic mice.

Abstract

The DNase I hypersensitive site 5' HS2 of the human beta-globin locus control region confers position-independent, high-level expression on the human beta-globin gene in transgenic mice. When a 5' HS2 beta-globin construct is flanked by retroviral vector sequences derived from Moloney Murine Leukemia Virus (MoMLV), expression of the beta-globin gene is severely inhibited. Apparently, one or more elements within the MoMLV genome is capable of repressing transcription of the human beta-globin gene in transgenic mice. A construct lacking the retroviral enhancer also fails to express the beta-globin gene, indicating that this region of the virus is not essential for repression. Further analysis may permit the identification of specific viral sequences that inhibit gene expression; these sequences could then be deleted or mutated to produce improved viral vectors.

Entities: Disease Gene Species

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Year: 1994 PMID： 7971278 PMCID： PMC308482 DOI： 10.1093/nar/22.21.4477

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

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Journal: Biotechniques Date: 1989-10 Impact factor: 1.993

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Journal: Nature Date: 1991-01-24 Impact factor: 49.962

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Journal: Proc Natl Acad Sci U S A Date: 1989-09 Impact factor: 11.205

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Journal: Nature Date: 1989-09-28 Impact factor: 49.962

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Journal: Proc Natl Acad Sci U S A Date: 1991-03-01 Impact factor: 11.205

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Authors: T D Palmer; G J Rosman; W R Osborne; A D Miller
Journal: Proc Natl Acad Sci U S A Date: 1991-02-15 Impact factor: 11.205

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Journal: Genes Dev Date: 1989-03 Impact factor: 11.361

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Journal: Proc Natl Acad Sci U S A Date: 1986-05 Impact factor: 11.205

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Journal: EMBO J Date: 1985-07 Impact factor: 11.598

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Journal: EMBO J Date: 1990-07 Impact factor: 11.598

11 in total

1. Synergism between hypersensitive sites confers long-range gene activation by the beta-globin locus control region.

Authors: E H Bresnick; L Tze
Journal: Proc Natl Acad Sci U S A Date: 1997-04-29 Impact factor: 11.205

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Journal: Nucleic Acids Res Date: 1997-03-15 Impact factor: 16.971

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Authors: W Y Chen; T M Townes
Journal: Proc Natl Acad Sci U S A Date: 2000-01-04 Impact factor: 11.205

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Authors: S Ghazizadeh; J M Carroll; L B Taichman
Journal: J Virol Date: 1997-12 Impact factor: 5.103

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Journal: J Virol Date: 1997-12 Impact factor: 5.103

6. Locus control region of the human CD2 gene in a lentivirus vector confers position-independent transgene expression.

Authors: C M Kowolik; J Hu; J K Yee
Journal: J Virol Date: 2001-05 Impact factor: 5.103

7. Amelioration of retroviral vector silencing in locus control region beta-globin-transgenic mice and transduced F9 embryonic cells.

Authors: C S Osborne; P Pasceri; R Singal; T Sukonnik; G D Ginder; J Ellis
Journal: J Virol Date: 1999-07 Impact factor: 5.103