Literature DB >> 10618426

Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.

W Y Chen1, T M Townes.   

Abstract

Virally transduced genes are often silenced after integration into the host genome. Chromatin immunoprecipitation and nuclease sensitivity experiments now demonstrate that silencing of the transgene is characterized by deacetylation of histone H4 lysines and chromatin condensation. Trichostatin A treatment results in dramatic reactivation of gene expression that is preceded by histone acetylation and chromatin decondensation. Analysis of individual histone H4 lysines demonstrate that chromatin domain opening is coincident with rapid acetylation of histone H4 K5, K12, and K16 and that maintenance of the open domain is correlated with acetylation of histone H4 K8. Removal of trichostatin A results in rapid deacetylation of histone H4 K8, chromatin condensation, and transcription silencing. The results suggest that deacetylation of histone H4 lysines and coincident chromatin condensation are critically involved in the silencing of virally transduced genes.

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Year:  2000        PMID： 10618426      PMCID： PMC26671          DOI： 10.1073/pnas.97.1.377

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

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Authors:  W C Forrester; E Epner; M C Driscoll; T Enver; M Brice; T Papayannopoulou; M Groudine
Journal:  Genes Dev       Date:  1990-10       Impact factor: 11.361

2.  DNA methylation models histone acetylation.

Authors:  S Eden; T Hashimshony; I Keshet; H Cedar; A W Thorne
Journal:  Nature       Date:  1998-08-27       Impact factor: 49.962

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Authors:  M J Pikaart; F Recillas-Targa; G Felsenfeld
Journal:  Genes Dev       Date:  1998-09-15       Impact factor: 11.361

4.  Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A.

Authors:  M Yoshida; M Kijima; M Akita; T Beppu
Journal:  J Biol Chem       Date:  1990-10-05       Impact factor: 5.157

5.  A novel, erythroid cell-specific murine transcription factor that binds to the CACCC element and is related to the Krüppel family of nuclear proteins.

Authors:  I J Miller; J J Bieker
Journal:  Mol Cell Biol       Date:  1993-05       Impact factor: 4.272

6.  Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.

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Journal:  Nature       Date:  1989-06-08       Impact factor: 49.962

7.  Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.

Authors:  M Braunstein; A B Rose; S G Holmes; C D Allis; J R Broach
Journal:  Genes Dev       Date:  1993-04       Impact factor: 11.361

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Authors:  T Evans; G Felsenfeld
Journal:  Cell       Date:  1989-09-08       Impact factor: 41.582

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Authors:  K Ajiro; T W Borun; L H Cohen
Journal:  Biochemistry       Date:  1981-03-17       Impact factor: 3.162

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Authors:  R Lin; J W Leone; R G Cook; C D Allis
Journal:  J Cell Biol       Date:  1989-05       Impact factor: 10.539
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  35 in total

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Journal:  Mol Cell Biol       Date:  2001-02       Impact factor: 4.272

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3.  Intergenic transcription in the human beta-globin gene cluster.

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Journal:  Mol Cell Biol       Date:  2001-10       Impact factor: 4.272

4.  Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci.

Authors:  M D Litt; M Simpson; F Recillas-Targa; M N Prioleau; G Felsenfeld
Journal:  EMBO J       Date:  2001-05-01       Impact factor: 11.598

5.  Activation of CMV promoter-controlled glycosyltransferase and beta -galactosidase glycogenes by butyrate, tricostatin A, and 5-aza-2'-deoxycytidine.

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Journal:  Glycoconj J       Date:  2005-02       Impact factor: 2.916

6.  An Erythroid-Specific Chromatin Opening Element Increases β-Globin Gene Expression from Integrated Retroviral Gene Transfer Vectors.

Authors:  Michael J Nemeth; Christopher H Lowrey
Journal:  Gene Ther Mol Biol       Date:  2004-12

7.  Np95 is implicated in pericentromeric heterochromatin replication and in major satellite silencing.

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8.  Blockade of histone deacetylase inhibitor-induced RelA/p65 acetylation and NF-kappaB activation potentiates apoptosis in leukemia cells through a process mediated by oxidative damage, XIAP downregulation, and c-Jun N-terminal kinase 1 activation.

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Journal:  Mol Cell Biol       Date:  2005-07       Impact factor: 4.272

9.  High-frequency epigenetic repression and silencing of retroviruses can be antagonized by histone deacetylase inhibitors and transcriptional activators, but uniform reactivation in cell clones is restricted by additional mechanisms.

Authors:  Richard A Katz; Emily Jack-Scott; Anna Narezkina; Ivan Palagin; Pamela Boimel; Joseph Kulkosky; Emmanuelle Nicolas; James G Greger; Anna Marie Skalka
Journal:  J Virol       Date:  2007-01-03       Impact factor: 5.103

10.  Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response.

Authors:  Andrey Poleshko; Ivan Palagin; Rugang Zhang; Pamela Boimel; Carolyn Castagna; Peter D Adams; Anna Marie Skalka; Richard A Katz
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