Literature DB >> 7956360

Instant PRINS: a rapid method for chromosome identification by detecting repeated sequences in situ.

J Gosden1, D Lawson.   

Abstract

We describe a method for labeling specific chromosomes in situ by oligonucleotide-primed synthesis and incorporation of fluorochrome-labeled dUTP, together with an accelerated protocol for the reaction. The combination of the two developments produces extremely rapid results, in some cases taking less than 5 min for the complete process. The significance of this development for clinical diagnosis is discussed.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7956360     DOI： 10.1159/000133889

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  3 in total

1.  Identification and quantitation of human chromosomes by primed in situ synthesis.

Authors:  J R Gosden
Journal:  Chromosome Res       Date:  1996-08       Impact factor: 5.239

2.  Multi-PRINS: multiple sequential oligonucleotide primed in situ DNA synthesis reactions label specific chromosomes and produce bands.

Authors:  E J Speel; D Lawson; A H Hopman; J Gosden
Journal:  Hum Genet       Date:  1995-01       Impact factor: 4.132

3.  High resolution multicolor fluorescence in situ hybridization using cyanine and fluorescein dyes: rapid chromosome identification by directly fluorescently labeled alphoid DNA probes.

Authors:  Y B Yurov; I V Soloviev; S G Vorsanova; B Marcais; G Roizes; R Lewis
Journal:  Hum Genet       Date:  1996-03       Impact factor: 4.132
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.