Literature DB >> 7814021

Multi-PRINS: multiple sequential oligonucleotide primed in situ DNA synthesis reactions label specific chromosomes and produce bands.

E J Speel1, D Lawson, A H Hopman, J Gosden.   

Abstract

A fast method for identifying several chromosomes with chromosome-specific oligonucleotide primers directing an in situ labeling reaction is described. Up to three reactions distinguished by different fluorochromes (fluorescein isothiocyanate, rhodamine/Texas red, p-amino-methyl-cyclohexane carboxylic acid) can currently be performed. Prospects for increasing this up to seven colors, and for the future of the process in prenatal diagnosis are discussed.

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Year:  1995        PMID： 7814021     DOI： 10.1007/bf00225069

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  9 in total

1.  Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy.

Authors:  T Ried; A Baldini; T C Rand; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205

2.  Multiple fluorescence in situ hybridization.

Authors:  P M Nederlof; S van der Flier; J Wiegant; A K Raap; H J Tanke; J S Ploem; M van der Ploeg
Journal:  Cytometry       Date:  1990

3.  PCR in situ: a rapid alternative to in situ hybridization for mapping short, low copy number sequences without isotopes.

Authors:  J Gosden; D Hanratty
Journal:  Biotechniques       Date:  1993-07       Impact factor: 1.993

4.  Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ labeling (PRINS).

Authors:  J Hindkjaer; J Koch; C Terkelsen; C A Brandt; S Kølvraa; L Bolund
Journal:  Cytogenet Cell Genet       Date:  1994

5.  Rapid chromosome identification by oligonucleotide-primed in situ DNA synthesis (PRINS).

Authors:  J Gosden; D Lawson
Journal:  Hum Mol Genet       Date:  1994-06       Impact factor: 6.150

6.  Instant PRINS: a rapid method for chromosome identification by detecting repeated sequences in situ.

Authors:  J Gosden; D Lawson
Journal:  Cytogenet Cell Genet       Date:  1995

7.  Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ.

Authors:  J E Koch; S Kølvraa; K B Petersen; N Gregersen; L Bolund
Journal:  Chromosoma       Date:  1989-10       Impact factor: 4.316

8.  A novel triple-color detection procedure for brightfield microscopy, combining in situ hybridization with immunocytochemistry.

Authors:  E J Speel; M P Jansen; F C Ramaekers; A H Hopman
Journal:  J Histochem Cytochem       Date:  1994-10       Impact factor: 2.479

9.  Oligonucleotide-primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding, and investigation of sequence organization.

Authors:  J Gosden; D Hanratty; J Starling; J Fantes; A Mitchell; D Porteous
Journal:  Cytogenet Cell Genet       Date:  1991
  9 in total
  3 in total

1.  Ultra-rapid multicolor PRINS protocol for chromosome detection in human sperm.

Authors:  F Pellestor; S Malki; B Andréo; G Lefort
Journal:  Chromosome Res       Date:  2002       Impact factor: 5.239

2.  Identification and quantitation of human chromosomes by primed in situ synthesis.

Authors:  J R Gosden
Journal:  Chromosome Res       Date:  1996-08       Impact factor: 5.239

Review 3.  Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors:  E J Speel; F C Ramaekers; A H Hopman
Journal:  Histochem J       Date:  1995-11
  3 in total

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