A morphometric analysis of the subcellular distribution of LH beta and FSH beta in secretory granules in the pituitary gonadotrophs of the frog (Rana japonica).

Immunohistochemical localization of lutropin beta (LH beta) and follitropin beta (FSH beta) in the pituitary gland of the frog Rana japonica was studied by the peroxidase-anti-peroxidase method and the two-face, double-labeling method with different-sized gold particles at the light- and electron-microscopic levels, respectively, using monoclonal antibodies against bullfrog LH beta and FSH beta. Light-microscopic immunohistochemistry indicated that approximately 66.0% of all the gonadotrophs in the pituitary contained both LH beta and FSH beta, whereas 33.4% of gonadotrophs contained only LH beta, and 0.6% contained only FSH beta. The staining intensity of LH beta and FSH beta varied from cell to cell. The gonadotrophs were classified into four types (Types I-IV) in terms of their ultrastructural and immunolabeling characteristics. Moreover, several secretory granule types were recognized according to differences in their shape and electron density. In all the cell types, both LH beta and FSH beta were often seen in the same secretory granules, but the proportion of granules bearing both hormones ranged from 5.5% in Type I to 32.7% in Type IV. Most secretory granules in Types I and II were immunolabeled with LH beta alone, whereas a small number of granules were immunolabeled with FSH beta alone. More immunolabeled FSH beta granules were present in Types III and IV than in Types I and II.