Immunohistochemical detection of adenohypophyseal cells containing hormones in Rana ridibunda.

The pars distalis of the pituitary of Rana ridibunda captured throughout the spring and summer was examined with immunofluorescence techniques using antisera to mammalian pituitary hormones. On the basis of their immunoreactivity, four different cell types of recognized: 1) cells immunoreactive to anti-bovine LTH, 2) cells immunoreactive to anti-bovine STH, 3) cells immunoreactive to anti-ovine LH, and 4) cells immunoreactive to anti-synthetic ACTH beta (1-24). Their distribution and morphology as well as their staining characteristics (classical histological techniques) are reported in this study.