OBJECTIVE: To develop a method to measure end expiratory nitric oxide concentration and to use it to determine whether there are sex related differences between healthy men and women. DESIGN: Modification of commercially available chemiluminescent analyser to allow endogenous nitric oxide (endothelium derived relaxing factor) to be measured in a single slow forced vital capacity manoeuvre with evaluation of between and within subject variability. Serial measurements in women throughout the menstrual cycle. SETTING: Specialist referral hospital. SUBJECTS: 59 healthy volunteers (40 men and 19 women). RESULTS: Mean (SD) peak expiratory concentrations of nitric oxide in men (75 (20) ppb (parts per billion)), and women (70 (37) ppb (first measurement)) were > 5 times those previously recorded. In women concentrations at midcycle (150(39) ppb) were significantly higher (mean difference 94.9(41), P < 0.001) than during menstruation (59(25) ppb). CONCLUSION: Nitric oxide production (endothelium derived relaxing factor activity) is influenced by cyclical hormonal changes in women. There was an increase of more than 100% at mid cycle. This may be pertinent to the lower risk of cardiovascular disease in premenopausal women.
OBJECTIVE: To develop a method to measure end expiratory nitric oxide concentration and to use it to determine whether there are sex related differences between healthy men and women. DESIGN: Modification of commercially available chemiluminescent analyser to allow endogenous nitric oxide (endothelium derived relaxing factor) to be measured in a single slow forced vital capacity manoeuvre with evaluation of between and within subject variability. Serial measurements in women throughout the menstrual cycle. SETTING: Specialist referral hospital. SUBJECTS: 59 healthy volunteers (40 men and 19 women). RESULTS: Mean (SD) peak expiratory concentrations of nitric oxide in men (75 (20) ppb (parts per billion)), and women (70 (37) ppb (first measurement)) were > 5 times those previously recorded. In women concentrations at midcycle (150(39) ppb) were significantly higher (mean difference 94.9(41), P < 0.001) than during menstruation (59(25) ppb). CONCLUSION:Nitric oxide production (endothelium derived relaxing factor activity) is influenced by cyclical hormonal changes in women. There was an increase of more than 100% at mid cycle. This may be pertinent to the lower risk of cardiovascular disease in premenopausal women.
Authors: T L Bush; E Barrett-Connor; L D Cowan; M H Criqui; R B Wallace; C M Suchindran; H A Tyroler; B M Rifkind Journal: Circulation Date: 1987-06 Impact factor: 29.690
Authors: Samar Farha; Kewal Asosingh; Daniel Laskowski; Lauren Licina; Haruki Sekiguchi; Haruki Sekigushi; Douglas W Losordo; Raed A Dweik; Herbert P Wiedemann; Serpil C Erzurum Journal: J Appl Physiol (1985) Date: 2007-08-23
Authors: Tze-Huan Lei; James D Cotter; Zachary J Schlader; Stephen R Stannard; Blake G Perry; Matthew J Barnes; Toby Mündel Journal: J Physiol Date: 2018-11-22 Impact factor: 5.182
Authors: Sze Man Tse; Sheryl L Rifas-Shiman; Brent A Coull; Augusto A Litonjua; Emily Oken; Diane R Gold Journal: J Allergy Clin Immunol Date: 2016-04-27 Impact factor: 10.793
Authors: Elizabeth A Townsend; Lucas W Meuchel; Michael A Thompson; Christina M Pabelick; Y S Prakash Journal: J Pharmacol Exp Ther Date: 2011-09-22 Impact factor: 4.030