Literature DB >> 7945238

Identification of one- and two-chain forms of trypsinogen 1 produced by a human gastric adenocarcinoma cell line.

N Koshikawa1, H Yasumitsu, Y Nagashima, M Umeda, K Miyazaki.   

Abstract

It has previously been reported that two kinds of human gastric adenocarcinoma cell lines (STKM-1 and MKN28) secrete a trypsin-like enzyme. In this study, four molecular forms of the enzyme (26, 25, 24 and 23 kDa on non-reducing SDS/PAGE) were purified from the serum-free conditioned medium of STKM-1 cells. Analysis of N-terminal amino acid sequences showed that the 26 kDa protein was a two-chain form of trypsinogen 1 which had been produced by proteolytic cleavage of the Arg107-Val108 bond of trypsinogen 1, and the 24 kDa protein was the one-chain form of trypsinogen 1. The 25 and 23 kDa proteins were the activated forms of the two-chain and one-chain trypsinogen 1 respectively. Isoelectric focusing gave pI values of 6.3 and 6.6 for the 26 kDa two-chain form and the 24 kDa one-chain form of trypsinogen 1 respectively. Comparison of the proteolytic activities indicated that the one-chain trypsin 1 had amidolytic activity about four times higher than that of the two-chain enzyme.

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Year:  1994        PMID： 7945238      PMCID： PMC1137574          DOI： 10.1042/bj3030187

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

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8.  Modulation of murine B16F10 melanoma plasminogen activator production by a synthetic peptide derived from the laminin A chain.

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9.  Two human trypsinogens. Purification, molecular properties, and N-terminal sequences.

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  8 in total

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7.  The levels of trypsinogen isoenzymes in ovarian tumour cyst fluids are associated with promatrix metalloproteinase-9 but not promatrix metalloproteinase-2 activation.

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8.  Characterization of matrix-degrading proteinases and their inhibitors secreted by human gynecological carcinoma cells.

Authors:  E Miyagi; H Yasumitsu; F Hirahara; H Minaguchi; N Koshikawa; K Miyazaki; M Umeda
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  8 in total

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