Literature DB >> 7944337

Selection of functional human immunoglobulin light chains from a phage-display library.

S Tyutyulkova1, S Paul.   

Abstract

Human kappa-light chains (L chains) were amplified by the reverse transcriptase-polymerase chain reaction (PCR) and cloned into a phagemid vector. Phage particles displaying L chains were fractionated on immobilized vasoactive intestinal peptide (VIP). The resultant phage preparation displayed saturable binding of (tyr10-125I)VIP. One of the L-chain clones (hk13) was deduced to be related to subgroup I of kappa-light chains based on its nucleotide sequence. The VIP binding activity of the soluble and phage-displayed form of this L chain was confirmed by radioimmunoassay and ELISA, respectively. These observations demonstrate the potential of selecting antigen-specific L chains from phage-display libraries.

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Year:  1994        PMID： 7944337     DOI： 10.1007/bf02787934

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  12 in total

1.  Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes.

Authors:  J D Marks; M Tristem; A Karpas; G Winter
Journal:  Eur J Immunol       Date:  1991-04       Impact factor: 5.532

2.  Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.

Authors:  H R Hoogenboom; A D Griffiths; K S Johnson; D J Chiswell; P Hudson; G Winter
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

3.  Mass, length, composition and structure of the filamentous bacterial virus fd.

Authors:  S A Berkowitz; L A Day
Journal:  J Mol Biol       Date:  1976-04-15       Impact factor: 5.469

4.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

5.  An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.

Authors:  S Munro; H R Pelham
Journal:  Cell       Date:  1986-07-18       Impact factor: 41.582

6.  Characterization of autoantibodies to vasoactive intestinal peptide in asthma.

Authors:  S Paul; S I Said; A B Thompson; D J Volle; D K Agrawal; H Foda; S de la Rocha
Journal:  J Neuroimmunol       Date:  1989-07       Impact factor: 3.478

7.  Antigen recognition by an antibody light chain.

Authors:  M Sun; L Li; Q S Gao; S Paul
Journal:  J Biol Chem       Date:  1994-01-07       Impact factor: 5.157

8.  Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody.

Authors:  S Paul; D J Volle; C M Beach; D R Johnson; M J Powell; R J Massey
Journal:  Science       Date:  1989-06-09       Impact factor: 47.728

9.  By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

Authors:  J D Marks; H R Hoogenboom; T P Bonnert; J McCafferty; A D Griffiths; G Winter
Journal:  J Mol Biol       Date:  1991-12-05       Impact factor: 5.469

10.  Activation of the alternative pathway of complement by monoclonal lambda light chains in membranoproliferative glomerulonephritis.

Authors:  S Meri; V Koistinen; A Miettinen; T Törnroth; I J Seppälä
Journal:  J Exp Med       Date:  1992-04-01       Impact factor: 14.307
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  1 in total

Review 1.  Natural catalytic antibodies.

Authors:  S Paul
Journal:  Mol Biotechnol       Date:  1996-06       Impact factor: 2.695
  1 in total

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