Literature DB >> 7937914

Dissociation of RecA filaments from duplex DNA by the RuvA and RuvB DNA repair proteins.

D E Adams1, I R Tsaneva, S C West.   

Abstract

The RuvA and RuvB proteins of Escherichia coli act late in recombination and DNA repair to catalyze the branch migration of Holliday junctions made by RecA. In this paper, we show that addition of RuvAB to supercoiled DNA that is bound by RecA leads to the rapid dissociation of the RecA nucleoprotein filament, as determined by a topological assay that measures DNA underwinding and a restriction endonuclease protection assay. Disruption of the RecA filament requires RuvA, RuvB, and hydrolysis of ATP. These findings suggest several important roles for the RuvAB helicase during genetic recombination and DNA repair: (i) displacement of RecA filaments from double-stranded DNA, (ii) interruption of RecA-mediated strand exchange, (iii) RuvAB-catalyzed branch migration, and (iv) recycling of RecA protein.

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Year:  1994        PMID： 7937914      PMCID： PMC44925          DOI： 10.1073/pnas.91.21.9901

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  In vitro replication through nucleosomes without histone displacement.

Authors:  C Bonne-Andrea; M L Wong; B M Alberts
Journal:  Nature       Date:  1990-02-22       Impact factor: 49.962

2.  Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination.

Authors:  G J Sharples; F E Benson; G T Illing; R G Lloyd
Journal:  Mol Gen Genet       Date:  1990-04

3.  Dissociation pathway for recA nucleoprotein filaments formed on linear duplex DNA.

Authors:  J E Lindsley; M M Cox
Journal:  J Mol Biol       Date:  1989-02-20       Impact factor: 5.469

4.  Stable binding of recA protein to duplex DNA. Unraveling a paradox.

Authors:  B F Pugh; M M Cox
Journal:  J Biol Chem       Date:  1987-01-25       Impact factor: 5.157

5.  recA protein binding to the heteroduplex product of DNA strand exchange.

Authors:  B F Pugh; M M Cox
Journal:  J Biol Chem       Date:  1987-01-25       Impact factor: 5.157

6.  The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA.

Authors:  A Stasiak; I R Tsaneva; S C West; C J Benson; X Yu; E H Egelman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-02       Impact factor: 11.205

7.  ATP-dependent unwinding of the double helix and extensive supercoiling by Escherichia coli recA protein in the presence of topoisomerase.

Authors:  M Iwabuchi; T Shibata; T Ohtani; M Natori; T Ando
Journal:  J Biol Chem       Date:  1983-10-25       Impact factor: 5.157

8.  Unwinding associated with synapsis of DNA molecules by recA protein.

Authors:  A M Wu; M Bianchi; C DasGupta; C M Radding
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

9.  Stimulation of protein-directed strand exchange by a DNA helicase.

Authors:  T Kodadek; B M Alberts
Journal:  Nature       Date:  1987 Mar 19-25       Impact factor: 49.962

10.  Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme).

Authors:  W S Dynan; J J Jendrisak; D A Hager; R R Burgess
Journal:  J Biol Chem       Date:  1981-06-10       Impact factor: 5.157
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  14 in total

1.  The Escherichia coli DinD protein modulates RecA activity by inhibiting postsynaptic RecA filaments.

Authors:  Lee A Uranga; Victoria D Balise; Candice V Benally; Angelina Grey; Shelley L Lusetti
Journal:  J Biol Chem       Date:  2011-06-22       Impact factor: 5.157

2.  A histone octamer blocks branch migration of a Holliday junction.

Authors:  M Grigoriev; P Hsieh
Journal:  Mol Cell Biol       Date:  1997-12       Impact factor: 4.272

Review 3.  Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.

Authors:  A Kuzminov
Journal:  Microbiol Mol Biol Rev       Date:  1999-12       Impact factor: 11.056

4.  Characterisation of RuvAB-Holliday junction complexes by glycerol gradient sedimentation.

Authors:  K Hiom; S C West
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

5.  Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair.

Authors:  Stefanie H Chen; Rose T Byrne; Elizabeth A Wood; Michael M Cox
Journal:  Mol Microbiol       Date:  2015-01-16       Impact factor: 3.501

6.  Deciphering Microbial Metal Toxicity Responses via Random Bar Code Transposon Site Sequencing and Activity-Based Metabolomics.

Authors:  Michael P Thorgersen; Jingchuan Xue; Erica L W Majumder; Valentine V Trotter; Xiaoxuan Ge; Farris L Poole; Trenton K Owens; Lauren M Lui; Torben N Nielsen; Adam P Arkin; Adam M Deutschbauer; Gary Siuzdak; Michael W W Adams
Journal:  Appl Environ Microbiol       Date:  2021-08-25       Impact factor: 4.792

7.  Homologous Recombination-Experimental Systems, Analysis, and Significance.

Authors:  Andrei Kuzminov
Journal:  EcoSal Plus       Date:  2011-12

8.  Characterization of the operon encoding the Holliday junction helicase RuvAB from Mycoplasma genitalium and its role in mgpB and mgpC gene variation.

Authors:  Raul Burgos; Patricia A Totten
Journal:  J Bacteriol       Date:  2014-02-14       Impact factor: 3.490

9.  Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.

Authors:  F E Benson; A Stasiak; S C West
Journal:  EMBO J       Date:  1994-12-01       Impact factor: 11.598

10.  The E.coli RuvAB proteins branch migrate Holliday junctions through heterologous DNA sequences in a reaction facilitated by SSB.

Authors:  C A Parsons; A Stasiak; S C West
Journal:  EMBO J       Date:  1995-11-15       Impact factor: 11.598
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