Literature DB >> 7928978

Effect of amino acid substitutions in a potential metal-binding site of AnfA on expression from the anfH promoter in Azotobacter vinelandii.

R Premakumar1, T M Loveless, P E Bishop.   

Abstract

AnfA, an activator required for transcription of the structural genes encoding nitrogenase 3 (anfHDGK) in Azotobacter vinelandii, has a potential metal-binding site [(S19)H(C21)FTGE(C26)R] in its N terminus. Growth studies and expression of an anfH-lacZ fusion in mutants containing amino acid substitutions in this site indicate that Ser-19 is not required for AnfA activity whereas Cys-21 and Cys-26 are required. Residual expression of the anfH-lacZ fusion in AnfA- mutants was found to be due to activation by VnfA, the activator required for expression of genes encoding nitrogenase 2.

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Year:  1994        PMID： 7928978      PMCID： PMC196836          DOI： 10.1128/jb.176.19.6139-6142.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  Transcriptional regulation by metals of structural genes for Azotobacter vinelandii nitrogenases.

Authors:  F Luque; R N Pau
Journal:  Mol Gen Genet       Date:  1991-07

2.  Nucleotide sequences and mutational analysis of the structural genes for nitrogenase 2 of Azotobacter vinelandii.

Authors:  R D Joerger; T M Loveless; R N Pau; L A Mitchenall; B H Simon; P E Bishop
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

3.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel; J D Roberts; R A Zakour
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii.

Authors:  L T Bennett; F Cannon; D R Dean
Journal:  Mol Microbiol       Date:  1988-05       Impact factor: 3.501

5.  Nitrogen fixation in molybdenum-deficient continuous culture by a strain of Azotobacter vinelandii carrying a deletion of the structural genes for nitrogenase (nifHDK).

Authors:  P E Bishop; M E Hawkins; R R Eady
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

6.  Characterization of transcripts expressed from nitrogenase-3 structural genes of Azotobacter vinelandii.

Authors:  R Premakumar; M R Jacobson; T M Loveless; P E Bishop
Journal:  Can J Microbiol       Date:  1992-09       Impact factor: 2.419

7.  Optimal conditions for transformation of Azotobacter vinelandii.

Authors:  W J Page; M von Tigerstrom
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

8.  Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii.

Authors:  R D Joerger; M R Jacobson; R Premakumar; E D Wolfinger; P E Bishop
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

9.  Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii.

Authors:  P E Bishop; D M Jarlenski; D R Hetherington
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

10.  Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

Authors:  M Drummond; P Whitty; J Wootton
Journal:  EMBO J       Date:  1986-02       Impact factor: 11.598
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  4 in total

1.  Azotobacter vinelandii vanadium nitrogenase: formaldehyde is a product of catalyzed HCN reduction, and excess ammonia arises directly from catalyzed azide reduction.

Authors:  Karl Fisher; Michael J Dilworth; William E Newton
Journal:  Biochemistry       Date:  2006-04-04       Impact factor: 3.162

2.  The hydrogenase cytochrome b heme ligands of Azotobacter vinelandii are required for full H(2) oxidation capability.

Authors:  L Meek; D J Arp
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

3.  Interactions between paralogous bacterial enhancer-binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii.

Authors:  Corinne Appia-Ayme; Richard Little; Govind Chandra; Carlo de Oliveira Martins; Marcelo Bueno Batista; Ray Dixon
Journal:  Mol Microbiol       Date:  2022-06-29       Impact factor: 3.979

4.  Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes.

Authors:  João C Setubal; Patricia dos Santos; Barry S Goldman; Helga Ertesvåg; Guadelupe Espin; Luis M Rubio; Svein Valla; Nalvo F Almeida; Divya Balasubramanian; Lindsey Cromes; Leonardo Curatti; Zijin Du; Eric Godsy; Brad Goodner; Kaitlyn Hellner-Burris; José A Hernandez; Katherine Houmiel; Juan Imperial; Christina Kennedy; Timothy J Larson; Phil Latreille; Lauren S Ligon; Jing Lu; Mali Maerk; Nancy M Miller; Stacie Norton; Ina P O'Carroll; Ian Paulsen; Estella C Raulfs; Rebecca Roemer; James Rosser; Daniel Segura; Steve Slater; Shawn L Stricklin; David J Studholme; Jian Sun; Carlos J Viana; Erik Wallin; Baomin Wang; Cathy Wheeler; Huijun Zhu; Dennis R Dean; Ray Dixon; Derek Wood
Journal:  J Bacteriol       Date:  2009-05-08       Impact factor: 3.490
  4 in total

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