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Literature DB >> 479104

Optimal conditions for transformation of Azotobacter vinelandii.

Abstract

Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated immediately on selective medium, but transformants acquiring rifampin and streptomycin resistance required preincubation in nonselective medium. The three phenotypes achieved an approximately equal and stable frequency after 17 h (six generations) of growth in nonselective medium.

Entities: Chemical Species

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Year: 1979 PMID： 479104 PMCID： PMC218056 DOI： 10.1128/jb.139.3.1058-1061.1979

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.

Authors: M S FOX; R D HOTCHKISS
Journal: Nature Date: 1960-09-17 Impact factor: 49.962

2. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

3. Intergeneric transfer of genes involved in the Rhizobium-legume symbiosis.

Authors: P E Bishop; F B Dazzo; E R Appelbaum; R J Maier; W J Brill
Journal: Science Date: 1977-12-02 Impact factor: 47.728

4. Factors affecting competence for transformation in Staphylococcus aureus.

Authors: L Rudin; J E Sjöström; M Lindberg; L Philipson
Journal: J Bacteriol Date: 1974-04 Impact factor: 3.490

5. Further observations on genetic transformation in pseudomonas.

Authors: N C Khan; S P Sen
Journal: J Gen Microbiol Date: 1974-08

6. Induction of transformation competence in Azotobacter vinelandii iron-limited cultures.

Authors: W J Page; M von Tigerstrom
Journal: Can J Microbiol Date: 1978-12 Impact factor: 2.419

7. Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen.

Authors: P E Bishop; W J Brill
Journal: J Bacteriol Date: 1977-05 Impact factor: 3.490

8. Transformation of Azotobacter vinelandii strains unable to fix nitrogen with Rhizobium spp. DNA.

Authors: W J Page
Journal: Can J Microbiol Date: 1978-03 Impact factor: 2.419

9. INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.

Authors: F E YOUNG; J SPIZIZEN
Journal: J Bacteriol Date: 1963-09 Impact factor: 3.490

10. BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS.

Authors: E W NESTER; B A STOCKER
Journal: J Bacteriol Date: 1963-10 Impact factor: 3.490

52 in total

1. A chromosomal linkage map of Azotobacter vinelandii.

Authors: G Blanco; F Ramos; J R Medina; M Tortolero
Journal: Mol Gen Genet Date: 1990-11

2. Regulation of nitrogenase-2 in Azotobacter vinelandii by ammonium, molybdenum, and vanadium.

Authors: S Jacobitz; P E Bishop
Journal: J Bacteriol Date: 1992-06 Impact factor: 3.490

3. Cloning and characterization of the Azotobacter vinelandii recA gene and construction of a recA deletion mutant.

Authors: T V Venkatesh; M A Reddy; H K Das
Journal: Mol Gen Genet Date: 1990-12

4. Hyperproduction of Poly-beta-Hydroxybutyrate during Exponential Growth of Azotobacter vinelandii UWD.

Authors: W J Page; O Knosp
Journal: Appl Environ Microbiol Date: 1989-06 Impact factor: 4.792

5. High Frequency of Natural Genetic Transformation of Pseudomonas stutzeri in Soil Extract Supplemented with a Carbon/Energy and Phosphorus Source.

Authors: M G Lorenz; W Wackernagel
Journal: Appl Environ Microbiol Date: 1991-04 Impact factor: 4.792

6. Temperature-Dependent Regulation by Molybdenum and Vanadium of Expression of the Structural Genes Encoding Three Nitrogenases in Azotobacter vinelandii.

Authors: J Walmsley; C Kennedy
Journal: Appl Environ Microbiol Date: 1991-02 Impact factor: 4.792

7. The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis.

Authors: Rhesa N Ledbetter; Amaya M Garcia Costas; Carolyn E Lubner; David W Mulder; Monika Tokmina-Lukaszewska; Jacob H Artz; Angela Patterson; Timothy S Magnuson; Zackary J Jay; H Diessel Duan; Jacquelyn Miller; Mary H Plunkett; John P Hoben; Brett M Barney; Ross P Carlson; Anne-Frances Miller; Brian Bothner; Paul W King; John W Peters; Lance C Seefeldt
Journal: Biochemistry Date: 2017-08-03 Impact factor: 3.162