| Literature DB >> 7926843 |
S E West1, H P Schweizer, C Dall, A K Sample, L J Runyen-Janecky.
Abstract
The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance of pMB1 (ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 beta-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.Entities:
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Year: 1994 PMID: 7926843 DOI: 10.1016/0378-1119(94)90237-2
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688