| Literature DB >> 7918687 |
H R Kim1, H W Rho, B H Park, J W Park, J S Kim, U H Kim, M Y Chung.
Abstract
Pretreatment of rats with verapamil, a Ca(2+)-antagonist, completely prevented alloxan-induced hyperglycemia. Verapamil also abolished the inhibition of insulin secretion by alloxan and H2O2 in isolated rat pancreatic islets. H2O2 generation from alloxan was not affected by verapamil, but alloxan- and H2O2-induced DNA strand breaks were completely prevented. Treatment of beta-cells with alloxan and H2O2 caused elevation of cytosolic free Ca2+, and this increase of Ca2+ was also abolished by verapamil. These results suggest that alloxan-derived oxygen radicals may disturb intracellular Ca2+ homeostasis by increasing Ca2+ influx, which results in secondary reactions ultimately leading to DNA strand breaks and cytotoxicity of beta-cells.Entities:
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Year: 1994 PMID： 7918687 DOI： 10.1016/0925-4439(94)90111-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002