Literature DB >> 791113

The substrate constant for the ammonium ion of growing Saccharomyces cerevisiae.

W J Middelhoven, M J Anderegg, A W Meijs, H P Egmond.   

Abstract

The relation between ammonium concentration and growth rate was studied in steady state continuous cultures of Saccharomyces cerevisiae in nitrogen-limited glucose ammonium medium. This relation could be described by the Monod equation. A maximum specific growth rate of 0.41 h-1 and a substrate constant for ammonium of 5-11 muM were calculated. Ammonium was determined by a modification of the phenol hypochlorite method. A discussion of the results in view of literature data on the substrate constants for other nutrients is given.

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Year:  1976        PMID: 791113     DOI: 10.1007/bf00394126

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN: 0003-6072            Impact factor:   2.271


  12 in total

1.  [SYNTHESIS OF NAD-DEPENDENT GLUTAMATE DEHYDROGENASE IN PROTOPLASTS OF SACCHAROMYCES CARLSBERGENSIS].

Authors:  H WESTPHAL; H HOLZER
Journal:  Biochim Biophys Acta       Date:  1964-07-08

2.  Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates.

Authors:  W E TREVELYAN; J S HARRISON
Journal:  Biochem J       Date:  1952-01       Impact factor: 3.857

3.  Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system.

Authors:  J J Gits; M Grenson
Journal:  Biochim Biophys Acta       Date:  1967-07-03

4.  Effect of nutrient concentration on the growth of Escherichia coli.

Authors:  T E Shehata; A G Marr
Journal:  J Bacteriol       Date:  1971-07       Impact factor: 3.490

5.  The cell size and macromolecular composition of Aerobacter aerogenes in various systems of continuous culture.

Authors:  A C Dean; P L Rogers
Journal:  Biochim Biophys Acta       Date:  1967-10-09

6.  Influence of the concentration of glucose and galactose on the physiology of Saccharomyces cerevisiae in continuous culture.

Authors:  C M Brown; B Johnson
Journal:  J Gen Microbiol       Date:  1970-12

7.  An extension of the theory of the chemostat with feedback of organisms. Its experimental realization with a yeast culture.

Authors:  S J Pirt; W M Kurowski
Journal:  J Gen Microbiol       Date:  1970-11

8.  Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system.

Authors:  M Grenson
Journal:  Biochim Biophys Acta       Date:  1966-10-31

9.  Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system.

Authors:  M Grenson; M Mousset; J M Wiame; J Bechet
Journal:  Biochim Biophys Acta       Date:  1966-10-31

10.  Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae.

Authors:  R J Roon; H L Even; F Larimore
Journal:  J Bacteriol       Date:  1974-04       Impact factor: 3.490

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  2 in total

1.  Nitrogen metabolite repression of arginase, ornithine transaminase and allantoinase in a conditional ethionine-resistant mutant of Saccharomyces cerevisiae with low activity of catabolic NAD-specific glutamate dehydrogenase.

Authors:  W J Middelhoven; M C Hoogkamer-te Niet
Journal:  Antonie Van Leeuwenhoek       Date:  1982-12       Impact factor: 2.271

2.  induction and derepression of arginase and ornithine transaminase in different strains of Saccharomyces cerevisiae.

Authors:  W J Middelhoven; G J Arkesteyn
Journal:  Antonie Van Leeuwenhoek       Date:  1981       Impact factor: 2.271

  2 in total

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