Literature DB >> 7891847

Solubilization and characterization of [3H]imipramine and [3H]paroxetine binding sites from calf striatum.

A Rotondo1, G Giannaccini, C Quattrone, D Marazziti, C Martini, G B Cassano, A Lucacchini.   

Abstract

The serotonin (5-HT) transporter from calf striatum cerebral membranes was solubilized with digitonin and characterized by gel exclusion chromatography. [3H]Imipramine and [3H]paroxetine were utilized as markers for labeling it. 3H-imipramine labels a high- and a low-affinity site on striatum membranes, whereas it binds to a single high-affinity site on the solubilized fraction. [3H]Paroxetine binds with the same affinity to a single site on both membranes and solubilized preparations. After gel exclusion chromatography of the solubilizate both [3H]imipramine and [3H]paroxetine bind on an identical fraction of 205 kDa molecular weight, with a similar maximum number of binding sites (Bmax). Our results suggest that both 3H-imipramine and [3H]paroxetine bind to a common site on the 5-HT transporter.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7891847     DOI： 10.1007/bf01006821

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  28 in total

1.  Cloning of a serotonin transporter affected by antidepressants.

Authors:  B J Hoffman; E Mezey; M J Brownstein
Journal:  Science       Date:  1991-10-25       Impact factor: 47.728

Review 2.  Biochemical hypotheses on antidepressant drugs: a guide for clinicians or a toy for pharmacologists?

Authors:  S Garattini; R Samanin
Journal:  Psychol Med       Date:  1988-05       Impact factor: 7.723

3.  Differentiation of two components of specific [3H]imipramine binding in rat brain.

Authors:  P D Hrdina
Journal:  Eur J Pharmacol       Date:  1984-07-20       Impact factor: 4.432

Review 4.  Binding of some antidepressants to the 5-hydroxytryptamine transporter in brain and platelets.

Authors:  J O Marcusson; S B Ross
Journal:  Psychopharmacology (Berl)       Date:  1990       Impact factor: 4.530

5.  Antidepressant- and cocaine-sensitive human serotonin transporter: molecular cloning, expression, and chromosomal localization.

Authors:  S Ramamoorthy; A L Bauman; K R Moore; H Han; T Yang-Feng; A S Chang; V Ganapathy; R D Blakely
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205

6.  Selective labeling of serotonin uptake sites in rat brain by [3H]citalopram contrasted to labeling of multiple sites by [3H]imipramine.

Authors:  R J D'Amato; B L Largent; A M Snowman; S H Snyder
Journal:  J Pharmacol Exp Ther       Date:  1987-07       Impact factor: 4.030

7.  Partial purification of the 5-hydroxytryptamine-reuptake system from human blood platelets using a citalopram-derived affinity resin [corrected].

Authors:  E A Biessen; A S Horn; G T Robillard
Journal:  Biochemistry       Date:  1990-04-03       Impact factor: 3.162

8.  High affinity binding of [3H]paroxetine and [3H]imipramine to human platelet membranes.

Authors:  E T Mellerup; P Plenge; M Engelstoft
Journal:  Eur J Pharmacol       Date:  1983-12-23       Impact factor: 4.432

9.  Isolation of a cDNA encoding the human brain serotonin transporter.

Authors:  K P Lesch; B L Wolozin; H C Estler; D L Murphy; P Riederer
Journal:  J Neural Transm Gen Sect       Date:  1993

10.  Dissociation kinetics of [3H]paroxetine binding to rat brain consistent with a single-site model of the antidepressant binding/5-hydroxytryptamine uptake site.

Authors:  J Marcusson; K Eriksson
Journal:  J Neurochem       Date:  1988-11       Impact factor: 5.372
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.