Literature DB >> 7887622

Detection of Escherichia coli O157:H7 in meat by an enzyme-linked immunosorbent assay, EHEC-Tek.

R P Johnson1, R J Durham, S T Johnson, L A MacDonald, S R Jeffrey, B T Butman.   

Abstract

Investigation of the specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHEC-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent, monoclonal antibody 4E8C12, were present in numerous serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA was improved by a modified test protocol incorporating immunocapture.

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Year:  1995        PMID： 7887622      PMCID： PMC167296          DOI： 10.1128/aem.61.1.386-388.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

Review 1.  The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome.

Authors:  P M Griffin; R V Tauxe
Journal:  Epidemiol Rev       Date:  1991       Impact factor: 6.222

2.  False-positive identification of Escherichia coli O157 by commercial latex agglutination tests.

Authors:  A A Borczyk; N Harnett; M Lombos; H Lior
Journal:  Lancet       Date:  1990-10-13       Impact factor: 79.321

3.  Characteristics of verotoxigenic Escherichia coli from pigs.

Authors:  V P Gannon; C L Gyles; R W Friendship
Journal:  Can J Vet Res       Date:  1988-07       Impact factor: 1.310

4.  Dipstick immunoassay to detect enterohemorrhagic Escherichia coli O157:H7 in retail ground beef.

Authors:  M S Kim; M P Doyle
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

5.  Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food.

Authors:  N V Padhye; M P Doyle
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

Review 6.  Infection by verocytotoxin-producing Escherichia coli.

Authors:  M A Karmali
Journal:  Clin Microbiol Rev       Date:  1989-01       Impact factor: 26.132

7.  Production and characterization of a monoclonal antibody specific for enterohemorrhagic Escherichia coli of serotypes O157:H7 and O26:H11.

Authors:  N V Padhye; M P Doyle
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948

8.  Vero cytotoxin-producing Escherichia coli, particularly serogroup O 157, associated with human infections in the United Kingdom: 1989-91.

Authors:  A Thomas; H Chart; T Cheasty; H R Smith; J A Frost; B Rowe
Journal:  Epidemiol Infect       Date:  1993-06       Impact factor: 2.451
  8 in total
  9 in total

1.  Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.

Authors:  W L Tsai; C E Miller; E R Richter
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

2.  Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method.

Authors:  Thomas R DeCory; Richard A Durst; Scott J Zimmerman; Linda A Garringer; Gary Paluca; Heleen H DeCory; Richard A Montagna
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

3.  Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli O157 in bovine fecal samples.

Authors:  P A Chapman; A T Malo; C A Siddons; M Harkin
Journal:  Appl Environ Microbiol       Date:  1997-07       Impact factor: 4.792

4.  Solid-phase capture of proteins, spores, and bacteria.

Authors:  B C Weimer; M K Walsh; C Beer; R Koka; X Wang
Journal:  Appl Environ Microbiol       Date:  2001-03       Impact factor: 4.792

Review 5.  Diarrheagenic Escherichia coli.

Authors:  J P Nataro; J B Kaper
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

6.  Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides.

Authors:  Manu S Mannoor; Siyan Zhang; A James Link; Michael C McAlpine
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-18       Impact factor: 11.205

7.  PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5' nuclease (TaqMan) assay.

Authors:  R D Oberst; M P Hays; L K Bohra; R K Phebus; C T Yamashiro; C Paszko-Kolva; S J Flood; J M Sargeant; J R Gillespie
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

8.  Monoclonal antibodies for detection of the H7 antigen of Escherichia coli.

Authors:  Y He; J E Keen; R B Westerman; E T Littledike; J Kwang
Journal:  Appl Environ Microbiol       Date:  1996-09       Impact factor: 4.792

9.  Development of a colony lift immunoassay to facilitate rapid detection and quantification of Escherichia coli O157:H7 from agar plates and filter monitor membranes.

Authors:  D T Ingram; C M Lamichhane; D M Rollins; L E Carr; E T Mallinson; S W Joseph
Journal:  Clin Diagn Lab Immunol       Date:  1998-07
  9 in total

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