Literature DB >> 7887464

Assay of ornithine aminotransferase with ninhydrin.

H R Kim1, H W Rho, J W Park, B H Park, J S Kim, M W Lee.   

Abstract

We developed an assay system for ornithine aminotransferase (EC 2.6.1.13) using ninhydrin. Pyrroline 5-carboxylate, a product of enzymatic transamination, reacts with ninhydrin under hot acidic conditions to form a reddish pigment soluble in ethanol. The millimolar extinction coefficient of reaction product dissolved in ethanol was 16.5 at 510 nm. Acidification with perchloric acid effectively abolished the interfering color development by L-ornithine and L-glutamate. The paired activity measurement in mouse tissues by ninhydrin and o-aminobenzaldehyde methods showed a good correlation (gamma = 0.985). In our ninhydrin method, stable ninhydrin replaced unstable o-aminobenzaldehyde, and sensitivity was much higher than that with the conventional o-aminobenzaldehyde method.

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Year:  1994        PMID： 7887464     DOI： 10.1006/abio.1994.1574

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  18 in total

1.  Effect of pH on spectral characteristics of P5C-ninhydrin derivative: Application in the assay of ornithine amino transferase activity from tissue lysate.

Authors:  H Ravikumar; K S Devaraju; K Taranath Shetty
Journal:  Indian J Clin Biochem       Date:  2008-06-11

2.  A sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method.

Authors:  H Ravi Kumar; S Ananda; K S Devaraju; B M Prakash; S Sampath Kumar; S V Suresh Babu; N Ramachandraswamy; H P Puttaraju
Journal:  Indian J Clin Biochem       Date:  2009-09-16

3.  Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.

Authors:  Salette Martinez; Robert P Hausinger
Journal:  Biochemistry       Date:  2016-10-21       Impact factor: 3.162

4.  Isolation of the ornithine-delta-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana.

Authors:  N H Roosens; T T Thu; H M Iskandar; M Jacobs
Journal:  Plant Physiol       Date:  1998-05       Impact factor: 8.340

5.  Effect of salinity on osmotic adjustment, proline accumulation and possible role of ornithine-δ-aminotransferase in proline biosynthesis in Cakile maritima.

Authors:  Dorsaf Hmidi; Chedly Abdelly; Habib-Ur-Rehman Athar; Muhammad Ashraf; Dorsaf Messedi
Journal:  Physiol Mol Biol Plants       Date:  2018-09-28

6.  ROS generation and proline metabolism in calli of halophyte Nitraria tangutorum Bobr. to sodium nitroprusside treatment.

Authors:  Fan Yang; Fan Ding; Xiaohui Duan; Jing Zhang; Xiaoning Li; Yingli Yang
Journal:  Protoplasma       Date:  2013-07-10       Impact factor: 3.356

7.  Arginase and C-reactive protein as potential serum-based biomarker of head and neck squamous cell carcinoma patients of north east India.

Authors:  Biswadeep Choudhury; Shilpee Srivastava; Haider H Choudhury; Alokananda Purkayastha; Sumita DuttaGupta; Sankar Kumar Ghosh
Journal:  Tumour Biol       Date:  2014-04-09

8.  Two continuous coupled assays for ornithine-δ-aminotransferase.

Authors:  Jose I Juncosa; Hyunbeom Lee; Richard B Silverman
Journal:  Anal Biochem       Date:  2013-06-05       Impact factor: 3.365

9.  Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.

Authors:  Rafael A Cañas; David P Villalobos; Sara M Díaz-Moreno; Francisco M Cánovas; Francisco R Cantón
Journal:  Plant Physiol       Date:  2008-07-11       Impact factor: 8.340

10.  Unraveling delta1-pyrroline-5-carboxylate-proline cycle in plants by uncoupled expression of proline oxidation enzymes.

Authors:  Gad Miller; Arik Honig; Hanan Stein; Nobuhiro Suzuki; Ron Mittler; Aviah Zilberstein
Journal:  J Biol Chem       Date:  2009-07-27       Impact factor: 5.157
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