Literature DB >> 7882137

The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.

A Choulika1, A Perrin, B Dujon, J F Nicolas.   

Abstract

Double-strand breaks in genomic DNA stimulate recombination. Until now it was not possible to induce in vivo site-directed double-strand breaks in a mammalian chromosomal target. In this article we describe the use of I-Sce I meganuclease, a very rare cutter yeast endonuclease, to induce site-directed double-strand breaks mediated recombination. The results demonstrate the potential of the I-Sce I system for chromosome manipulation in mammalian cells.

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Year:  1994        PMID： 7882137

Source DB:  PubMed          Journal:  C R Acad Sci III        ISSN： 0764-4469


  13 in total

1.  Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.

Authors:  Rafael Molina; Pilar Redondo; Blanca López-Méndez; Maider Villate; Nekane Merino; Francisco J Blanco; Julien Valton; Silvestre Grizot; Phillipe Duchateau; Jesús Prieto; Guillermo Montoya
Journal:  J Biol Chem       Date:  2015-09-11       Impact factor: 5.157

2.  Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.

Authors:  Pilar Redondo; Jesús Prieto; Elena Ramos; Francisco J Blanco; Guillermo Montoya
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-11-21

3.  Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cre gene and the loxP site.

Authors:  A Choulika; V Guyot; J F Nicolas
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

4.  Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA.

Authors:  Pilar Redondo; Nekane Merino; Maider Villate; Francisco J Blanco; Guillermo Montoya; Rafael Molina
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-01-22       Impact factor: 1.056

5.  I-SceI-induced gene replacement at a natural locus in embryonic stem cells.

Authors:  M Cohen-Tannoudji; S Robine; A Choulika; D Pinto; F El Marjou; C Babinet; D Louvard; F Jaisser
Journal:  Mol Cell Biol       Date:  1998-03       Impact factor: 4.272

6.  Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis.

Authors:  Eduard Renfer; Ulrich Technau
Journal:  Nat Protoc       Date:  2017-08-17       Impact factor: 13.491

Review 7.  Oligo/polynucleotide-based gene modification: strategies and therapeutic potential.

Authors:  R Geoffrey Sargent; Soya Kim; Dieter C Gruenert
Journal:  Oligonucleotides       Date:  2011-03-21

8.  Structure of the I-SceI nuclease complexed with its dsDNA target and three catalytic metal ions.

Authors:  Jesús Prieto; Pilar Redondo; Nekane Merino; Maider Villate; Guillermo Montoya; Francisco J Blanco; Rafael Molina
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2016-05-23       Impact factor: 1.056

9.  Single and multiple gene knockouts by CRISPR-Cas9 in maize.

Authors:  Nicolas M Doll; Laurine M Gilles; Marie-France Gérentes; Christelle Richard; Jeremy Just; Yannick Fierlej; Virginia M G Borrelli; Ghislaine Gendrot; Gwyneth C Ingram; Peter M Rogowsky; Thomas Widiez
Journal:  Plant Cell Rep       Date:  2019-01-25       Impact factor: 4.570

10.  I-SceI-mediated double-strand break does not increase the frequency of homologous recombination at the Dct locus in mouse embryonic stem cells.

Authors:  Myriam Fenina; Dominique Simon-Chazottes; Sandrine Vandormael-Pournin; Jihane Soueid; Francina Langa; Michel Cohen-Tannoudji; Bruno A Bernard; Jean-Jacques Panthier
Journal:  PLoS One       Date:  2012-06-26       Impact factor: 3.240
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