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Literature DB >> 787936

Arangement of transfer-RNA -genes in yeast.

Abstract

The redundancy and the arrangement of the genes for specific transfer ribonucleic acids in yeast were studied by the hybridization techniques developed by Birnstiel et al., e.g.[1]. The redundancy was found to be in the order of 10 genes for tRNA1Met, tRNA3Met, tRNA2Ser, and tRNA-Pro. High molecular weight yeast DNA was fractionated by density gradient centrifugation in cesium chloride and the [32p]tRNAs were hybridized to the single fractions. The results together with earlier findings [2] suggest that the cistrons for these tRNAs are arranged in tandem interspersed by 6 to 10 times longer segments of spacer DNA which varies in (G+C) content for the different tRNA species.

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Year: 1976 PMID： 787936 PMCID： PMC343092 DOI： 10.1093/nar/3.9.2379

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

Arangement of transfer-RNA -genes in yeast.

1. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.

2. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.

3. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

4. Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae.

5. Characterization of precursors to tRNA in yeast.

6. The primary structure of yeast initiator transfer ribonucleic acid.

7. Clustering of transfer RNA genes of Xenopus laevis.

8. A two-dimensional fractionation procedure for radioactive nucleotides.

9. Kinetic complexity of RNA molecules.

10. Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro.

1. Putative frameshift suppressors in Schizosaccharomyces pombe.

2. A comparison of transcriptional linkage of tRNA cistrons in yeast and E. coli by the ultraviolet light technique.

3. Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.

4. Genes and pseudogenes in a reiterated rat tRNA gene cluster.

5. Isolation and characterization of cloned rat DNA fragment carrying tRNA genes.

6. Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene.

7. Mammalian tRNA genes: nucleotide sequence of rat genes for tRNAAsp, tRNAGly and tRNAGlu.

8. Structural comparison of two yeast tRNA Glu 3 genes.

9. Structure of a yeast non-initiating methionine-tRNA gene.

10. Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae.