Literature DB >> 787763

Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.

W van Alphen, B Lugtenberg, W Berendsen.   

Abstract

Mutants of Escherichia coli K12, deficient in up to three major outer membrane proteins b, c and d have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in protein b. All heptose-deficient strains are supersensitive to lysozyme, various antibiotics and detergents. They excrete the periplasmic enzyme ribonuclease I. Mutants deficient in proteins c and/or d have the same sensitivity towards these compounds as the parent strain. Cells of single, double and triple mutants are all rod-shaped. Electrophoretic analysis of cell envelope proteins indicates that in some mutants the protein deficiency is partially compensated for by increased amounts of one or two of the other major outer membrane proteins. Heptose-deficient strains have an increased amount of 2-keto-3-deoxyoctonate.

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Year:  1976        PMID： 787763     DOI： 10.1007/bf00582877

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  35 in total

1.  STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM.

Authors:  M J OSBORN
Journal:  Proc Natl Acad Sci U S A       Date:  1963-09       Impact factor: 11.205

2.  Demonstration of a missing outer membrane protein in tolG mutants of Escherichia cell.

Authors:  T Chai; J Foulds
Journal:  J Mol Biol       Date:  1974-05-25       Impact factor: 5.469

3.  Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors:  M J Osborn; J E Gander; E Parisi; J Carson
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

4.  Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.

Authors:  C A Schnaitman
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490

5.  Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure.

Authors:  V Braun; K Rehn
Journal:  Eur J Biochem       Date:  1969-10

6.  Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide.

Authors:  L M Havekes; B J Lugtenberg; W P Hoekstra
Journal:  Mol Gen Genet       Date:  1976-07-05

7.  Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli.

Authors:  B Lugtenberg; R Peters; H Bernheimer; W Berendsen
Journal:  Mol Gen Genet       Date:  1976-09-23

8.  Increase in sensitivity to antibiotics and lysozyme on deletion of lipopolysaccharides in Escherichia coli strains.

Authors:  S Tamaki; M Matsuhashi
Journal:  J Bacteriol       Date:  1973-04       Impact factor: 3.490

9.  Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.

Authors:  G F Ames; E N Spudich; H Nikaido
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

10.  Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli.

Authors:  J Koplow; H Goldfine
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490
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  29 in total

Review 1.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

2.  Pores in the outer membrane of Escherichia coli K12: involvement of proteins b and e in the functioning of pores for nucleotides.

Authors:  W van Alphen; N van Seim; B Lugtenberg
Journal:  Mol Gen Genet       Date:  1978-02-07

3.  Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria.

Authors:  J de Jongh-Leuvenink; A S Bouter; J H Marcelis; J Schellekens; J Verhoef
Journal:  Eur J Clin Microbiol       Date:  1986-04       Impact factor: 3.267

4.  Isolation and characterization of Escherichia coli K-12 F- mutants defective in conjugation with an I-type donor.

Authors:  L Havekes; J Tommassen; W Hoekstra; B Lugtenberg
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

5.  Outer membrane of Serratia marcescens: apparent molecular weights of heat-modifiable proteins in gels with different acrylamide concentrations.

Authors:  K B Heller
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

6.  Genetic and biochemical characterization of an Escherichia coli K-12 mutant with an altered outer membrane protein.

Authors:  J Tommassen; P van der Ley; B Lugtenberg
Journal:  Antonie Van Leeuwenhoek       Date:  1981       Impact factor: 2.271

7.  Mapping of a gene for a major outer membrane protein of Escherichia coli K12 with the aid of a newly isolated bacteriophage.

Authors:  C Verhoef; P J de Graaff; E J Lugtenberg
Journal:  Mol Gen Genet       Date:  1977-01-07

8.  Cytochemical localization of lipopolysaccharides during peptidoglycan degradation of Escherichia coli cells.

Authors:  C Frehel; M Leduc
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

9.  Architecture of the outer membrane of Escherichia coli. III. Protein-lipopolysaccharide complexes in intramembraneous particles.

Authors:  L van Alphen; A Verkleij; J Leunissen-Bijvelt; B Lugtenberg
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

10.  Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens.

Authors:  U Winkler; K B Heller; B Folle
Journal:  Arch Microbiol       Date:  1978-03       Impact factor: 2.552
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