E E Takahashi1, C L Rossi. 1. Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.
Abstract
AIMS: To assess the performance and efficacy of three immunological techniques for the detection of Toxoplasma specific IgA antibodies in acute toxoplasmosis. METHODS: The following techniques were used to examine 128 serum samples (51 cases of acute toxoplasmosis, 50 cases of heterologous infections, and 27 healthy controls): direct enzyme linked immunosorbent assay (ELISA), antibody capture ELISA, and antibody capture agglutination. RESULTS: Direct ELISA had a sensitivity of 98% and a specificity of 97%, antibody capture ELISA of 100% and 99%, respectively, and antibody capture agglutination had sensitivity and specificity of 100%. CONCLUSIONS: All three immunological techniques performed well with similar efficacy. Detection of Toxoplasma specific IgA antibodies is a useful diagnostic marker for acute toxoplasmosis.
AIMS: To assess the performance and efficacy of three immunological techniques for the detection of Toxoplasma specific IgA antibodies in acute toxoplasmosis. METHODS: The following techniques were used to examine 128 serum samples (51 cases of acute toxoplasmosis, 50 cases of heterologous infections, and 27 healthy controls): direct enzyme linked immunosorbent assay (ELISA), antibody capture ELISA, and antibody capture agglutination. RESULTS: Direct ELISA had a sensitivity of 98% and a specificity of 97%, antibody capture ELISA of 100% and 99%, respectively, and antibody capture agglutination had sensitivity and specificity of 100%. CONCLUSIONS: All three immunological techniques performed well with similar efficacy. Detection of Toxoplasma specific IgA antibodies is a useful diagnostic marker for acute toxoplasmosis.
Authors: Alexandre C de Macedo; Juliana A Guimarães; Raphael O Rodrigues; Thiago D V Araújo; Clodis M Tavares; Paula B Cabral; Maria Isabel de Moraes-Pinto; Aparecida T Nagao-Dias Journal: J Clin Lab Anal Date: 2017-06-08 Impact factor: 2.352
Authors: Tudor Rares Olariu; Brian G Blackburn; Cindy Press; Jeanne Talucod; Jack S Remington; Jose G Montoya Journal: J Clin Microbiol Date: 2019-01-30 Impact factor: 5.948