Alexandre C de Macedo1, Juliana A Guimarães1, Raphael O Rodrigues1, Thiago D V Araújo2, Clodis M Tavares3, Paula B Cabral4, Maria Isabel de Moraes-Pinto5, Aparecida T Nagao-Dias1. 1. Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, Universidade Federal do Ceará- UFC, Fortaleza, Brazil. 2. Department of Pharmacy, Faculty of Pharmacy, UFC, Fortaleza, Brazil. 3. School of Nursery and Pharmacy (ESENFAR), Universidade Federal de Alagoas, Maceió, Brazil. 4. Division of Pharmacy, Hospital Universitário Walter Cantídio, Universidade Federal do Ceara, Fortaleza, Brazil. 5. Division of Pediatric Infectious Diseases, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Abstract
OBJECTIVE: The aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. METHOD: Analysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. RESULTS: A strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%; 95% confidence interval) and 38.7% (24.4%-54.5%); for IgG, 100% (87.0%-100.0%) and 28.8% (17.8%-42.1%), respectively; and for IgM, 95.2% (83.8%-99.4%) and 51.7% (32.5%-70.6%), respectively. CONCLUSION: Despite the limiting factors, anti-PGL1 IgA correlates to IgM levels and it could be considered as a possible laboratorial tool to be also used, for instance, in serological follow-up studies.
OBJECTIVE: The aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosypatients and controls. METHOD: Analysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosypatients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. RESULTS: A strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MBpatients. A moderate correlation was found in all analyses in PBpatients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%; 95% confidence interval) and 38.7% (24.4%-54.5%); for IgG, 100% (87.0%-100.0%) and 28.8% (17.8%-42.1%), respectively; and for IgM, 95.2% (83.8%-99.4%) and 51.7% (32.5%-70.6%), respectively. CONCLUSION: Despite the limiting factors, anti-PGL1 IgA correlates to IgM levels and it could be considered as a possible laboratorial tool to be also used, for instance, in serological follow-up studies.
Authors: Aparecida T Nagao-Dias; Thereza Lúcia P Almeida; Maria de Fátima Oliveira; Rivanda C Santos; Ana Laura P Lima; Márcia Brasil Journal: Braz J Infect Dis Date: 2007-04 Impact factor: 1.949
Authors: Paula Brito e Cabral; José Evandro Cunha Júnior; Alexandre Casimiro de Macedo; Alexandre Rodrigues Alves; Thially Braga Gonçalves; Tereza Cristina Brito e Cabral; Ana Paula Soares Gondim; Maria Isabel Moraes Pinto; Karen Tubono Oseki; Lilia Maria Carneiro Camara; Silvia Helena Barem Rabenhorst; Aparecida Tiemi Nagao-Dias Journal: Int J Infect Dis Date: 2013-07-16 Impact factor: 3.623
Authors: Kyssia Karen de Paiva E Silva; Erick Esteves de Oliveira; Carolina Martins Moreira Elias; Ingrid Estevam Pereira; Roberta Olmo Pinheiro; Euzenir Nunes Sarno; Malcolm Scott Duthie; Henrique Couto Teixeira Journal: Front Med (Lausanne) Date: 2021-08-10