Literature DB >> 7875576

Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945.

L Martín1, M A Prieto, E Cortés, J L García.   

Abstract

The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 (proA, leuB) using a selective minimal medium containing phenylacetyl-L-leucine instead of L-leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other beta-lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.

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Year:  1995        PMID： 7875576     DOI： 10.1016/0378-1097(94)00510-x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  9 in total

1.  The PaaX repressor, a link between penicillin G acylase and the phenylacetyl-coenzyme A catabolon of Escherichia coli W.

Authors:  Beatriz Galán; José L García; María A Prieto
Journal:  J Bacteriol       Date:  2004-04       Impact factor: 3.490

2.  Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis.

Authors:  R M Verhaert; A M Riemens; J M van der Laan; J van Duin; W J Quax
Journal:  Appl Environ Microbiol       Date:  1997-09       Impact factor: 4.792

3.  Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration.

Authors:  Catleya Rojviriya; Thunyaluck Pratumrat; Mark A Saper; Jirundon Yuvaniyama
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-26

4.  Classification of two species of Gram-positive bacteria through hyperspectral microscopy coupled with machine learning.

Authors:  Kunxing Liu; Ze Ke; Peining Chen; Siqi Zhu; Hao Yin; Zhen Li; Zhenqiang Chen
Journal:  Biomed Opt Express       Date:  2021-12-01       Impact factor: 3.732

5.  Role of alphaArg145 and betaArg263 in the active site of penicillin acylase of Escherichia coli.

Authors:  Wynand B L Alkema; Antoon K Prins; Erik de Vries; Dick B Janssen
Journal:  Biochem J       Date:  2002-07-01       Impact factor: 3.857

6.  Protein engineering of penicillin acylase.

Authors:  V I Tishkov; S S Savin; A S Yasnaya
Journal:  Acta Naturae       Date:  2010-07       Impact factor: 1.845

7.  High-throughput strategies for penicillin G acylase production in rE. coli fed-batch cultivations.

Authors:  Ana Maria Vélez; Adilson José da Silva; Antonio Carlos Luperni Horta; Cintia Regina Sargo; Gilson Campani; Gabriel Gonçalves Silva; Raquel de Lima Camargo Giordano; Teresa Cristina Zangirolami
Journal:  BMC Biotechnol       Date:  2014-01-21       Impact factor: 2.563

8.  Genomic Analysis of Bacillus megaterium NCT-2 Reveals Its Genetic Basis for the Bioremediation of Secondary Salinization Soil.

Authors:  Bin Wang; Dan Zhang; Shaohua Chu; Yuee Zhi; Xiaorui Liu; Pei Zhou
Journal:  Int J Genomics       Date:  2020-02-28       Impact factor: 2.326

9.  Manually curated genome-scale reconstruction of the metabolic network of Bacillus megaterium DSM319.

Authors:  Javad Aminian-Dehkordi; Seyyed Mohammad Mousavi; Arezou Jafari; Ivan Mijakovic; Sayed-Amir Marashi
Journal:  Sci Rep       Date:  2019-12-10       Impact factor: 4.379
  9 in total

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