Literature DB >> 7870582

Molecular recognition of tRNA(Pro) by Escherichia coli proline tRNA synthetase in vitro.

H Liu1, R Peterson, J Kessler, K Musier-Forsyth.   

Abstract

In this study, we identify a subset of nucleotides that specify aminoacylation of tRNA(Pro) by Escherichia coli proline tRNA synthetase in vitro. Twenty-two tRNA(Pro) variants were prepared by in vitro transcription and their efficiency of aminoacylation with proline (kcat/KM) was measured. From this analysis, we conclude that recognition elements for tRNA(Pro) aminoacylation by ProRS are located in at least three domains of the tRNA molecule. The largest decreases in the kinetic parameters for aminoacylation resulted from single substitutions at position G72 of the acceptor stem and position G36 of the anticodon. Anticodon nucleotide G35 and position A73 in the acceptor stem were also identified as major recognition elements. Moreover, bases that are believed to be important for maintaining the tertiary structure of the tRNA (G15 and C48) appear to be important for efficient recognition of tRNA(Pro) by ProRS in vitro.

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Year:  1995        PMID： 7870582      PMCID： PMC306645          DOI： 10.1093/nar/23.1.165

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  Escherichia coli proline tRNA synthetase is sensitive to changes in the core region of tRNA(Pro).

Authors:  H Liu; K Musier-Forsyth
Journal:  Biochemistry       Date:  1994-10-25       Impact factor: 3.162

Review 2.  The transfer RNA identity problem: a search for rules.

Authors:  M E Saks; J R Sampson; J N Abelson
Journal:  Science       Date:  1994-01-14       Impact factor: 47.728

3.  Changing the identity of a transfer RNA.

Authors:  J Normanly; R C Ogden; S J Horvath; J Abelson
Journal:  Nature       Date:  1986 May 15-21       Impact factor: 49.962

4.  Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases.

Authors:  A R Fersht; J S Ashford; C J Bruton; R Jakes; G L Koch; B S Hartley
Journal:  Biochemistry       Date:  1975-01-14       Impact factor: 3.162

Review 5.  Rules that govern tRNA identity in protein synthesis.

Authors:  W H McClain
Journal:  J Mol Biol       Date:  1993-11-20       Impact factor: 5.469

6.  Distinctive acceptor-end structure and other determinants of Escherichia coli tRNAPro identity.

Authors:  W H McClain; J Schneider; K Gabriel
Journal:  Nucleic Acids Res       Date:  1994-02-11       Impact factor: 16.971

7.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

8.  Nucleotide sequences of three proline tRNAs from Salmonella typhimurium.

Authors:  Y Kuchino; Y Yabusaki; F Mori; S Nishimura
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

9.  Nucleoside modifications stabilize Mg2+ binding in Escherichia coli tRNA(Val): an imino proton NMR investigation.

Authors:  D Yue; A Kintanar; J Horowitz
Journal:  Biochemistry       Date:  1994-08-02       Impact factor: 3.162

10.  The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser).

Authors:  V Biou; A Yaremchuk; M Tukalo; S Cusack
Journal:  Science       Date:  1994-03-11       Impact factor: 47.728
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  20 in total

1.  Crystal structure of a eukaryote/archaeon-like protyl-tRNA synthetase and its complex with tRNAPro(CGG).

Authors:  A Yaremchuk; S Cusack; M Tukalo
Journal:  EMBO J       Date:  2000-09-01       Impact factor: 11.598

2.  tRNA integrity is a prerequisite for rapid CCA addition: implication for quality control.

Authors:  Marcel Dupasquier; Sangbumn Kim; Konstantine Halkidis; Howard Gamper; Ya-Ming Hou
Journal:  J Mol Biol       Date:  2008-04-08       Impact factor: 5.469

3.  Activation of microhelix charging by localized helix destabilization.

Authors:  R W Alexander; B E Nordin; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-13       Impact factor: 11.205

4.  Effect of a mutation in the anticodon of human mitochondrial tRNAPro on its post-transcriptional modification pattern.

Authors:  H Brulé; W M Holmes; G Keith; R Giegé; C Florentz
Journal:  Nucleic Acids Res       Date:  1998-01-15       Impact factor: 16.971

5.  Hydrolytic editing by a class II aminoacyl-tRNA synthetase.

Authors:  P J Beuning; K Musier-Forsyth
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

6.  Restoring species-specific posttransfer editing activity to a synthetase with a defunct editing domain.

Authors:  Julius SternJohn; Sanchita Hati; Paul G Siliciano; Karin Musier-Forsyth
Journal:  Proc Natl Acad Sci U S A       Date:  2007-02-05       Impact factor: 11.205

7.  Mg2+ binding and archaeosine modification stabilize the G15 C48 Levitt base pair in tRNAs.

Authors:  Romina Oliva; Anna Tramontano; Luigi Cavallo
Journal:  RNA       Date:  2007-07-24       Impact factor: 4.942

8.  Human trans-editing enzyme displays tRNA acceptor-stem specificity and relaxed amino acid selectivity.

Authors:  Oscar Vargas-Rodriguez; Marina Bakhtina; Daniel McGowan; Jawad Abid; Yuki Goto; Hiroaki Suga; Karin Musier-Forsyth
Journal:  J Biol Chem       Date:  2020-10-13       Impact factor: 5.157

9.  Functional guanine-arginine interaction between tRNAPro and prolyl-tRNA synthetase that couples binding and catalysis.

Authors:  Brian Burke; Songon An; Karin Musier-Forsyth
Journal:  Biochim Biophys Acta       Date:  2008-05-10

10.  Exclusive use of trans-editing domains prevents proline mistranslation.

Authors:  Oscar Vargas-Rodriguez; Karin Musier-Forsyth
Journal:  J Biol Chem       Date:  2013-04-05       Impact factor: 5.157
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