Literature DB >> 7866873

Lectin affinity chromatography.

I West, O Goldring.   

Abstract

A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyldiimidazole-activated agarose are described.

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Year:  1994        PMID： 7866873     DOI： 10.1007/bf02824807

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  30 in total

1.  Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.

Authors:  S P Jackson; R Tjian
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

2.  Isolation and partial characterization of papain-solubilized murine H-2 antigens.

Authors:  S Kvist; L Sandberg-Trägårdh; L Ostberg; P A Peterson
Journal:  Biochemistry       Date:  1977-10-04       Impact factor: 3.162

3.  The purification of detergent-solubilized HL-A antigens by affinity chromatography with the hemagglutinin from Lens culinaris.

Authors:  J R Dawson; J Silver; L B Sheppard; D B Amos
Journal:  J Immunol       Date:  1974-03       Impact factor: 5.422

4.  Glycoprotein purification on a high-capacity wheat germ lectin affinity column.

Authors:  G Mintz; L Glaser
Journal:  Anal Biochem       Date:  1979-09-01       Impact factor: 3.365

5.  Schistosoma mansoni: surface membrane isolation with lectin-coated beads.

Authors:  F H Pujol; I M Cesari
Journal:  Membr Biochem       Date:  1993 Jul-Sep

6.  Affinity chromatography of cells and cell membranes.

Authors:  S K Sharma; P P Mahendroo
Journal:  J Chromatogr       Date:  1980-11-07

7.  A simple and effective solvent system for elution of gonadotropins from concanavalin A affinity chromatography.

Authors:  S Matsuura; H C Chen
Journal:  Anal Biochem       Date:  1980-08       Impact factor: 3.365

8.  Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells.

Authors:  V Kinzel; D Kübler; J Richards; M Stöhr
Journal:  Science       Date:  1976-04-30       Impact factor: 47.728

9.  Turnover and orientation of the major neural retina cell surface protein protected from tryptic cleavage by calcium.

Authors:  J H Cook; R S Pratt; J Lilien
Journal:  Biochemistry       Date:  1984-02-28       Impact factor: 3.162

10.  Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.

Authors:  R Lotan; G Beattie; W Hubbell; G L Nicolson
Journal:  Biochemistry       Date:  1977-05-03       Impact factor: 3.162
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