Literature DB >> 1257785

Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells.

V Kinzel, D Kübler, J Richards, M Stöhr.   

Abstract

Lens culinaris lectin (LCL) covalently linked to 2B Sepharose binds tissue culture cells to the matrix. This is prevented by hapten sugars specific for LCL. Unlike other immobilized lectins, lens culinaris lectin allows the removal of bound cells from the matrix on addition of the specific sugars in a concentration-dependent manner. Binding and release occur under physiological conditions. Released cells continue to grow.

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Year:  1976        PMID： 1257785     DOI： 10.1126/science.1257785

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  3 in total

1.  Protein-coated agarose surfaces for attachment of cells.

Authors:  J Carlsson; D Gabel; E Larsson; J Pontén; B Westermark
Journal:  In Vitro       Date:  1979-11

2.  Lectin affinity chromatography.

Authors:  I West; O Goldring
Journal:  Mol Biotechnol       Date:  1994-10       Impact factor: 2.695

3.  A versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations.

Authors:  M E Pereira; E A Kabat
Journal:  J Cell Biol       Date:  1979-07       Impact factor: 10.539
  3 in total

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