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Literature DB >> 786199

Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy.

Abstract

The beta(1-3)glucanase of Basidiomycete QM 806 was used to prepare Saccharomyces cerevisiae and Candida utilis protoplasts. Plasma membranes isolated from S. cerevisiae contained a small amount of mannose and traces of glucose and ribose. Randomly distributed alpha-mannan was detected by scanning electron microscopy at the surface of prefixed protoplasts using colloidal gold labelled with Concanavalin A as a marker. C. utilis protoplasts were also marked with anti-mannan antibodies. Again the distribution of mannan was random. This experiment indicated also that plasma membrane mannan has the same immunochemical determinants as cell wall mannan. It is hypothesized that mannan is mainly located in the outer layer of plasma membranes.

Entities: Chemical Species

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Year: 1976 PMID： 786199 DOI： 10.1007/bf00425106

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

17 in total

3. Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.

Authors: M Horiseberger; J Rosset
Journal: Arch Microbiol Date: 1977-03-01 Impact factor: 2.552

Review 4. A review of cell surface markers and labelling techniques for scanning electron microscopy.

Authors: R S Molday; P Maher
Journal: Histochem J Date: 1980-05

5. Location of mannan and chitin on thin sections of budding yeasts with gold markers.

Authors: M Horisberger; M Vonlanthen
Journal: Arch Microbiol Date: 1977-10-24 Impact factor: 2.552

6. Regeneration of the cell wall in protoplasts of Candida albicans. A cytochemical study using wheat germ agglutinin and concanavalin A.

Authors: F Miragall; H Rico; R Sentandreu
Journal: Arch Microbiol Date: 1988 Impact factor: 2.552

6 in total

Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy.

1. Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

2. Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans.

3. PROTEIN-CARBOHYDRATE INTERACTION. I. THE INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A.

4. Mannan as a major component of the bud scars of Saccharomyces cerevisiae.

5. Use of the exo- -(1 leads to 3) glucanase from basidiomycete QM 806 in studies on yeast.

6. Site of mannan synthesis in yeast. An autoradiographic study.

7. Isolation and identification of yeast plasma membrane.

8. Purification of an exo-beta-D-(1 bonded to 3)-glucanase from Basidiomycete species QM 806.

9. Composition of the protoplast membrane from Saccharomyces cerevisiae.

10. Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

1. Immunolocalization of two lignin O-methyltransferases in stems of alfalfa (Medicago sativa L.).

Review 2. Immunogold labeling in scanning electron microscopy.

3. Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.

Review 4. A review of cell surface markers and labelling techniques for scanning electron microscopy.

5. Location of mannan and chitin on thin sections of budding yeasts with gold markers.

6. Regeneration of the cell wall in protoplasts of Candida albicans. A cytochemical study using wheat germ agglutinin and concanavalin A.