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Literature DB >> 4123394

Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

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Year: 1973 PMID： 4123394 PMCID： PMC422705 DOI： 10.1128/iai.7.3.487-492.1973

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

12 in total

7. Studies on the antigenic activities of yeasts. II. Isolation and inhibition assay of the oligosaccharides from acetolysate of the mannan of Candida albicans.

Authors: S Suzuki; H Sunayama
Journal: Jpn J Microbiol Date: 1968-12

3. Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.

Authors: M Horiseberger; J Rosset
Journal: Arch Microbiol Date: 1977-03-01 Impact factor: 2.552

Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

1. Comparison of the effects of various detergents on antigen-antibody interaction.

2. Mercury-labelled concanavalin A as a marker in electron microscopy-localisation of mannan in yeast cell walls.

3. Mannan as a major component of the bud scars of Saccharomyces cerevisiae.

4. Entrapment of collagen in a polyacrylamide matrix and its application in the purification of animal collagenases.

5. An immunocolloid method for the electron microscope.

6. Preparation of polyacrylamide gels which contain protein and their use as high capacity immunosorbents.

7. Studies on the antigenic activities of yeasts. II. Isolation and inhibition assay of the oligosaccharides from acetolysate of the mannan of Candida albicans.

8. A study of the immunochemistry of three yeast mannans.

9. Method for fingerprinting yeast cell wall mannans.

10. ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS.

1. Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

2. Morphology of colloidal gold, ferritin and anti-ferritin antibody complexes.

3. Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.

4. Ultrastructural localization of cell wall teichoic acids in Streptococcus faecalis by means of concanavalin A.

5. Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy.

6. Location of mannan and chitin on thin sections of budding yeasts with gold markers.