Literature DB >> 7856852

Simultaneous measurement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence plate reader.

S W Kennedy1, S P Jones.   

Abstract

A method for simultaneous measurement of the cytochrome P4501A-associated enzyme, ethoxyresorufin-O-deethylase (EROD), and total protein concentration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and total proteins were quantified within the same wells with a fluorescence plate reader. Fluorescamine was used for protein assays.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7856852     DOI： 10.1006/abio.1994.1476

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  39 in total

1.  Measurement of EROD activity: caution on spectral properties of standards used.

Authors:  G Radenac; G Coteur; B Danis; Ph Dubois; M Warnau
Journal:  Mar Biotechnol (NY)       Date:  2004-07-30       Impact factor: 3.619

2.  A novel contact assay for testing aryl hydrocarbon receptor (AhR)-mediated toxicity of chemicals and whole sediments in zebrafish (Danio rerio) embryos.

Authors:  Sabrina Schiwy; Jennifer Bräunig; Henriette Alert; Henner Hollert; Steffen H Keiter
Journal:  Environ Sci Pollut Res Int       Date:  2014-06-25       Impact factor: 4.223

3.  High-throughput enzyme assay on a multichannel microchip using optically gated sample introduction.

Authors:  Hongwei Xu; Andrew G Ewing
Journal:  Electrophoresis       Date:  2005-12       Impact factor: 3.535

4.  Toxicity, dioxin-like activities, and endocrine effects of DDT metabolites--DDA, DDMU, DDMS, and DDCN.

Authors:  Bernhard Wetterauer; Mathias Ricking; Jens C Otte; Arnold V Hallare; Andrew Rastall; Lothar Erdinger; Jan Schwarzbauer; Thomas Braunbeck; Henner Hollert
Journal:  Environ Sci Pollut Res Int       Date:  2011-07-27       Impact factor: 4.223

5.  A microassay for the detection of low levels of cytochrome P450 O-deethylation activities with alkoxyresorufin substrates.

Authors:  J Lagueux; E B Affar; D Nadeau; P Ayotte; E Dewailly; G G Poirier
Journal:  Mol Cell Biochem       Date:  1997-10       Impact factor: 3.396

6.  Protective effect of ellagic acid against TCDD-induced renal oxidative stress: modulation of CYP1A1 activity and antioxidant defense mechanisms.

Authors:  Viswanadha Vijaya Padma; Palaniswamy Kalai Selvi; Samadi Sravani
Journal:  Mol Biol Rep       Date:  2014-02-25       Impact factor: 2.316

7.  Hepatic EROD activity is not a useful biomarker of polychlorinated biphenyl exposure in the adult herring gull (Larus argentatus).

Authors:  Sean W Kennedy; Glen A Fox; Stephanie P Jones; Suzanne F Trudeau
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

8.  Biological effects of marine PCB contamination on black guillemot nestlings at Saglek, Labrador: liver biomarkers.

Authors:  Zou Zou A Kuzyk; Neil M Burgess; Jason P Stow; Glen A Fox
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

9.  Developmental exposure to polychlorinated biphenyls interferes with experience-dependent dendritic plasticity and ryanodine receptor expression in weanling rats.

Authors:  Dongren Yang; Kyung Ho Kim; Andrew Phimister; Adam D Bachstetter; Thomas R Ward; Robert W Stackman; Ronald F Mervis; Amy B Wisniewski; Sabra L Klein; Prasada Rao S Kodavanti; Kim A Anderson; Gary Wayman; Isaac N Pessah; Pamela J Lein
Journal:  Environ Health Perspect       Date:  2008-09-12       Impact factor: 9.031

10.  Hepatic P450 enzyme activity, tissue morphology and histology of mink (Mustela vison) exposed to polychlorinated dibenzofurans.

Authors:  Jeremy N Moore; John L Newsted; Markus Hecker; Matthew J Zwiernik; Scott D Fitzgerald; Denise P Kay; Xiaowei Zhang; Eric B Higley; Lesa L Aylward; Kerrie J Beckett; Robert A Budinsky; Steven J Bursian; John P Giesy
Journal:  Arch Environ Contam Toxicol       Date:  2009-05-21       Impact factor: 2.804
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.