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Literature DB >> 7843103

Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.

Abstract

A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp domain of hprt exon 3. Substitutions at G:C base pairs were the predominant mutations. One of the chromium-induced hotspots was located at the same position as previously determined hydrogen peroxide and benzo(a)pyrene diol epoxide hotspots.

Entities: Chemical Species

Mesh：

Substances：
Chromium

Year: 1994 PMID： 7843103 PMCID： PMC1567402 DOI： 10.1289/ehp.94102s3227

Source DB: PubMed Journal: Environ Health Perspect ISSN： 0091-6765 Impact factor: 9.031

8 in total

1. Mutational spectrometry: a general approach for hot-spot point mutations in selectable genes.

Authors: P Keohavong; W G Thilly
Journal: Proc Natl Acad Sci U S A Date: 1992-05-15 Impact factor: 11.205

2. Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases.

Authors: L L Ling; P Keohavong; C Dias; W G Thilly
Journal: PCR Methods Appl Date: 1991-08

3. Molecular basis of base substitution hotspots in Escherichia coli.

Authors: C Coulondre; J H Miller; P J Farabaugh; W Gilbert
Journal: Nature Date: 1978-08-24 Impact factor: 49.962

4. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.

Authors: C Coulondre; J H Miller
Journal: J Mol Biol Date: 1977-12-15 Impact factor: 5.469

5. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

Authors: S G Fischer; L S Lerman
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

6. Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.

Authors: R P Fuchs; N Schwartz; M P Daune
Journal: Nature Date: 1981-12-17 Impact factor: 49.962

7. Structural intermediates of deletion mutagenesis: a role for palindromic DNA.

Authors: B W Glickman; L S Ripley
Journal: Proc Natl Acad Sci U S A Date: 1984-01 Impact factor: 11.205

8. Mutational spectra in human B-cells. Spontaneous, oxygen and hydrogen peroxide-induced mutations at the hprt gene.

Authors: A R Oller; W G Thilly
Journal: J Mol Biol Date: 1992-12-05 Impact factor: 5.469

8 in total

4 in total

Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.

1. Mutational spectrometry: a general approach for hot-spot point mutations in selectable genes.

2. Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases.

3. Molecular basis of base substitution hotspots in Escherichia coli.

4. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.

5. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

6. Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.

7. Structural intermediates of deletion mutagenesis: a role for palindromic DNA.

8. Mutational spectra in human B-cells. Spontaneous, oxygen and hydrogen peroxide-induced mutations at the hprt gene.

1. Single nucleotide polymorphism seeking long term association with complex disease.

2. DNA polymerase zeta is essential for hexavalent chromium-induced mutagenesis.

3. Analysis of mutational spectra by denaturing capillary electrophoresis.

4. Differential impact of ionic and coordinate covalent chromium (Cr)-DNA binding on DNA replication.