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Literature DB >> 7819499

Condensation of supercoiled DNA induced by MnCl2.

Abstract

Multivalent cations condense DNA in vitro, but it had been thought that a valence of at least + 3 was required in aqueous solution. We have found that Mn2+ can produce toroidal condensates of supercoiled plasmid DNA, but not of linearized plasmid. Mg2+ does not cause condensation, and neither MgCl2 nor NaCl can negate the effect of MnCl2, indicating that the condensation mechanism with Mn is not primarily electrostatic. Supercoiled MnDNA is more extensively digested than the linear form by S1 nuclease. Supercoiling appears to cooperate with Mn2+ in stabilizing helix distortions and also provides a "pressure" that enhances lateral association.

Entities: Chemical

Mesh：

Substances：

Year: 1994 PMID： 7819499 PMCID： PMC1225529 DOI： 10.1016/S0006-3495(94)80641-1

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

31 in total

Review 1. Supercoiled DNA and cruciform structures.

Authors: A I Murchie; D M Lilley
Journal: Methods Enzymol Date: 1992 Impact factor: 1.600

2. Condensation of DNA by trivalent cations. 1. Effects of DNA length and topology on the size and shape of condensed particles.

Authors: P G Arscott; A Z Li; V A Bloomfield
Journal: Biopolymers Date: 1990 Impact factor: 2.505

3. Structure of plectonemically supercoiled DNA.

Authors: T C Boles; J H White; N R Cozzarelli
Journal: J Mol Biol Date: 1990-06-20 Impact factor: 5.469

4. Rate of B to Z structural transition of supercoiled DNA.

Authors: L J Peck; J C Wang; A Nordheim; A Rich
Journal: J Mol Biol Date: 1986-07-05 Impact factor: 5.469

5. Interaction of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity.

Authors: G L Eichhorn; Y A Shin
Journal: J Am Chem Soc Date: 1968-12-18 Impact factor: 15.419

6. The twist, writhe and overall shape of supercoiled DNA change during counterion-induced transition from a loosely to a tightly interwound superhelix. Possible implications for DNA structure in vivo.

Authors: J Bednar; P Furrer; A Stasiak; J Dubochet; E H Egelman; A D Bates
Journal: J Mol Biol Date: 1994-01-21 Impact factor: 5.469

7. B-DNA to Z-DNA structural transitions in the SV40 enhancer: stabilization of Z-DNA in negatively supercoiled DNA minicircles.

Authors: E A Gruskin; A Rich
Journal: Biochemistry Date: 1993-03-09 Impact factor: 3.162

8. Kinetics of cruciform formation and stability of cruciform structure in superhelical DNA.

Authors: I Panyutin; V Klishko; V Lyamichev
Journal: J Biomol Struct Dyn Date: 1984-06

9. Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd.

Authors: J Duguid; V A Bloomfield; J Benevides; G J Thomas
Journal: Biophys J Date: 1993-11 Impact factor: 4.033

10. Direct visualization of supercoiled DNA molecules in solution.

Authors: M Adrian; B ten Heggeler-Bordier; W Wahli; A Z Stasiak; A Stasiak; J Dubochet
Journal: EMBO J Date: 1990-12 Impact factor: 11.598

21 in total

1. Brownian dynamics simulation of DNA condensation.

Authors: P E Sottas; E Larquet; A Stasiak; J Dubochet
Journal: Biophys J Date: 1999-10 Impact factor: 4.033

2. Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences.

Authors: G L Wilson; B S Dean; G Wang; D A Dean
Journal: J Biol Chem Date: 1999-07-30 Impact factor: 5.157

3. Multiscale study of counterion-induced attraction and bundle formation of F-actin using an Ising-like mean-field model.

Authors: Xueping Yu; A E Carlsson
Journal: Biophys J Date: 2003-12 Impact factor: 4.033

Condensation of supercoiled DNA induced by MnCl2.

Review 1. Supercoiled DNA and cruciform structures.

2. Condensation of DNA by trivalent cations. 1. Effects of DNA length and topology on the size and shape of condensed particles.

3. Structure of plectonemically supercoiled DNA.

4. Rate of B to Z structural transition of supercoiled DNA.

5. Interaction of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity.

6. The twist, writhe and overall shape of supercoiled DNA change during counterion-induced transition from a loosely to a tightly interwound superhelix. Possible implications for DNA structure in vivo.

7. B-DNA to Z-DNA structural transitions in the SV40 enhancer: stabilization of Z-DNA in negatively supercoiled DNA minicircles.

8. Kinetics of cruciform formation and stability of cruciform structure in superhelical DNA.

9. Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd.

10. Direct visualization of supercoiled DNA molecules in solution.

1. Brownian dynamics simulation of DNA condensation.

2. Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences.

3. Multiscale study of counterion-induced attraction and bundle formation of F-actin using an Ising-like mean-field model.

4. Azimuthal frustration and bundling in columnar DNA aggregates.

Review 5. DNA toroids: framework for DNA repair in Deinococcus radiodurans and in germinating bacterial spores.

6. Hexamminecobalt(III)-induced condensation of calf thymus DNA: circular dichroism and hydration measurements.

7. DNA nano- and microparticles: new products of polymerase chain reaction.

8. Rapid and efficient technique for the production of condensed DNA and RNA nanoparticles using thermal cycling.

Review 9. Polyelectrolyte properties of filamentous biopolymers and their consequences in biological fluids.

Review 10. Importance of diffuse metal ion binding to RNA.