Literature DB >> 7819342

Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells.

F M de Bree1, J P Burbach.   

Abstract

To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.

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Year:  1994        PMID： 7819342     DOI： 10.1016/0300-9084(94)90165-1

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  5 in total

1.  Structural Requirements for Sorting Pro-Vasopressin to the Regulated Secretory Pathway in a Neuronal Cell Line.

Authors:  David R Cool; Steven B Jackson; Karen S Waddell
Journal:  Open Neuroendocrinol J       Date:  2008-01-01

2.  In search of dihydrofolate reductase.

Authors:  F M Huennekens
Journal:  Protein Sci       Date:  1996-06       Impact factor: 6.725

Review 3.  Structure-function relationships of the vasopressin prohormone domains.

Authors:  F M de Bree; J P Burbach
Journal:  Cell Mol Neurobiol       Date:  1998-04       Impact factor: 5.046

4.  Molecular basis of autosomal dominant neurohypophyseal diabetes insipidus. Cellular toxicity caused by the accumulation of mutant vasopressin precursors within the endoplasmic reticulum.

Authors:  M Ito; J L Jameson; M Ito
Journal:  J Clin Invest       Date:  1997-04-15       Impact factor: 14.808

5.  EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells.

Authors:  Bing-Jun Zhang; Mitsuo Yamashita; Ray Fields; Kiyoshi Kusano; Harold Gainer
Journal:  Cell Mol Neurobiol       Date:  2005-06       Impact factor: 5.046
  5 in total

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