Literature DB >> 7818508

Anti-syntaxin antibodies inhibit calcium-dependent catecholamine secretion from permeabilized chromaffin cells.

L M Gutierrez1, J L Quintanar, S Viniegra, E Salinas, F Moya, J A Reig.   

Abstract

Adrenomedullary chromaffin cells release catecholamines in response to the intracellular calcium rise upon stimulation by different secretagogues. The presence of syntaxin 1, a protein presumably involved in docking of synaptic vesicles to presynaptic membranes, has been investigated in chromaffin cells. The study using two different monoclonal antibodies shows that syntaxin 1 is present in the chromaffin cell membrane fraction. Functional experiments demonstrate that anti-syntaxin antibodies inhibit calcium-dependent secretion in permeabilized cells. These results suggest that syntaxin 1 is an important component of the secretory machinery in chromaffin cells.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7818508     DOI： 10.1006/bbrc.1995.1001

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  Inhibition of insulin release by synthetic peptides shows that the H3 region at the C-terminal domain of syntaxin-1 is crucial for Ca(2+)- but not for guanosine 5'-[gamma-thio]triphosphate-induced secretion.

Authors:  F Martin; E Salinas; J Vazquez; B Soria; J A Reig
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

2.  Characterization of HPC-1 antigen, an isoform of syntaxin-1, with the isoform-specific monoclonal antibody, 14D8.

Authors:  Y Kushima; T Fujiwara; M Sanada; K Akagawa
Journal:  J Mol Neurosci       Date:  1997-02       Impact factor: 3.444

3.  Role of syntaxin in mouse pancreatic beta cells.

Authors:  F Martín; F Moya; L M Gutierrez; J A Reig; B Soria
Journal:  Diabetologia       Date:  1995-07       Impact factor: 10.122

4.  Syntaxin 4 in 3T3-L1 adipocytes: regulation by insulin and participation in insulin-dependent glucose transport.

Authors:  A Volchuk; Q Wang; H S Ewart; Z Liu; L He; M K Bennett; A Klip
Journal:  Mol Biol Cell       Date:  1996-07       Impact factor: 4.138

Review 5.  The use of permeabilized cells to assay protein phosphorylation and catecholamine release.

Authors:  C A Gonçalves; C Gottfried; P R Dunkley
Journal:  Neurochem Res       Date:  2000-06       Impact factor: 3.996

6.  F-actin-myosin II inhibitors affect chromaffin granule plasma membrane distance and fusion kinetics by retraction of the cytoskeletal cortex.

Authors:  José Villanueva; Vanesa Torres; Cristina J Torregrosa-Hetland; Virginia Garcia-Martinez; Inmaculada López-Font; Salvador Viniegra; Luis M Gutiérrez
Journal:  J Mol Neurosci       Date:  2012-05-17       Impact factor: 3.444

7.  Platelet granule exocytosis: a comparison with chromaffin cells.

Authors:  Jennifer L Fitch-Tewfik; Robert Flaumenhaft
Journal:  Front Endocrinol (Lausanne)       Date:  2013-06-26       Impact factor: 5.555

8.  The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells.

Authors:  Yolanda Gimenez-Molina; José Villanueva; Carmen Nanclares; Inmaculada Lopez-Font; Salvador Viniegra; Maria Del Mar Francés; Luis Gandia; Amparo Gil; Luis M Gutiérrez
Journal:  Front Cell Neurosci       Date:  2017-05-04       Impact factor: 5.505

9.  Multiple sclerosis drug FTY-720 toxicity is mediated by the heterotypic fusion of organelles in neuroendocrine cells.

Authors:  Yolanda Gimenez-Molina; Virginia García-Martínez; José Villanueva; Bazbek Davletov; Luis M Gutiérrez
Journal:  Sci Rep       Date:  2019-12-05       Impact factor: 4.379

10.  Multiple Mechanisms Driving F-actin-Dependent Transport of Organelles to and From Secretory Sites in Bovine Chromaffin Cells.

Authors:  Yolanda Gimenez-Molina; José Villanueva; Maria Del Mar Francés; Salvador Viniegra; Luis M Gutiérrez
Journal:  Front Cell Neurosci       Date:  2018-10-09       Impact factor: 5.505
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.