Literature DB >> 7814342

Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus.

M Rolfsmeier1, P Blum.   

Abstract

A soluble maltase (alpha-glucosidase) with an apparent subunit mass of 80 kDa was purified to homogeneity from Sulfolobus solfataricus. The enzyme liberates glucose from maltose and malto-oligomers. Maximal activity was observed at 105 degrees C, with half-lives of 11 h (85 degrees C), 3.0 h (95 degrees C), and 2.75 h (100 degrees C). The enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-Propanol treatment at 85 degrees C increased both Km and Vmax for maltose hydrolysis.

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Year:  1995        PMID： 7814342      PMCID： PMC176616          DOI： 10.1128/jb.177.2.482-485.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus.

Authors:  S H Brown; H R Costantino; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1990-07       Impact factor: 4.792

2.  Nucleotide sequence of the 16S rRNA gene from thermoacidophilic archaea Sulfolobus acidocaldarius ATCC33909.

Authors:  N Kurosawa; Y H Itoh
Journal:  Nucleic Acids Res       Date:  1993-01-25       Impact factor: 16.971

3.  Confusion in the assignments of Sulfolobus sequences to Sulfolobus species.

Authors:  W Zillig
Journal:  Nucleic Acids Res       Date:  1993-11-11       Impact factor: 16.971

4.  Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature.

Authors:  T D Brock; K M Brock; R T Belly; R L Weiss
Journal:  Arch Mikrobiol       Date:  1972

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The purification and characterization of an extremely thermostable alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus.

Authors:  K A Laderman; B R Davis; H C Krutzsch; M S Lewis; Y V Griko; P L Privalov; C B Anfinsen
Journal:  J Biol Chem       Date:  1993-11-15       Impact factor: 5.157

7.  Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.

Authors:  D A Hager; R R Burgess
Journal:  Anal Biochem       Date:  1980-11-15       Impact factor: 3.365

8.  Extremely thermophilic acidophilic bacteria convergent with Sulfolobus acidocaldarius.

Authors:  M de Rosa; A Gambacorta; J D Bu'lock
Journal:  J Gen Microbiol       Date:  1975-01

9.  Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115 degrees C.

Authors:  H R Costantino; S H Brown; R M Kelly
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

10.  Phenotypic characterization of the archaebacterial genus Sulfolobus: comparison of five wild-type strains.

Authors:  D W Grogan
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490
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  34 in total

1.  Coordinate transcriptional control in the hyperthermophilic archaeon Sulfolobus solfataricus.

Authors:  C Haseltine; R Montalvo-Rodriguez; E Bini; A Carl; P Blum
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

2.  Extragenic pleiotropic mutations that repress glycosyl hydrolase expression in the hyperthermophilic archaeon Sulfolobus solfataricus.

Authors:  C Haseltine; R Montalvo-Rodriguez; A Carl; E Bini; P Blum
Journal:  Genetics       Date:  1999-08       Impact factor: 4.562

3.  Large-scale cultivation of acidophilic hyperthermophiles for recovery of secreted proteins.

Authors:  Penny Worthington; Paul Blum; Francisco Perez-Pomares; Tom Elthon
Journal:  Appl Environ Microbiol       Date:  2003-01       Impact factor: 4.792

Review 4.  The unique features of glycolytic pathways in Archaea.

Authors:  Corné H Verhees; Servé W M Kengen; Judith E Tuininga; Gerrit J Schut; Michael W W Adams; Willem M De Vos; John Van Der Oost
Journal:  Biochem J       Date:  2003-10-15       Impact factor: 3.857

5.  The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfolobus solfataricus.

Authors:  Viet Hoang; Elisabetta Bini; Vidula Dixit; Melissa Drozda; Paul Blum
Journal:  Genetics       Date:  2004-08       Impact factor: 4.562

6.  Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution.

Authors:  Samuel McCarthy; Tyler Johnson; Benjamin J Pavlik; Sophie Payne; Wendy Schackwitz; Joel Martin; Anna Lipzen; Erica Keffeler; Paul Blum
Journal:  Appl Environ Microbiol       Date:  2015-11-20       Impact factor: 4.792

7.  Characterization of different crystal forms of the alpha-glucosidase MalA from Sulfolobus solfataricus.

Authors:  Heidi Asschenfeldt Ernst; Martin Willemoës; Leila Lo Leggio; Gordon Leonard; Paul Blum; Sine Larsen
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-11-05

8.  Membrane-bound amylopullulanase is essential for starch metabolism of Sulfolobus acidocaldarius DSM639.

Authors:  Kyoung-Hwa Choi; Jaeho Cha
Journal:  Extremophiles       Date:  2015-06-24       Impact factor: 2.395

9.  Nonmutational mechanism of inheritance in the Archaeon Sulfolobus solfataricus.

Authors:  Sophie Payne; Samuel McCarthy; Tyler Johnson; Erica North; Paul Blum
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-13       Impact factor: 11.205

10.  An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA.

Authors:  Cynthia A Haseltine; Stephen C Kowalczykowski
Journal:  Nucleic Acids Res       Date:  2009-03-12       Impact factor: 16.971
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