Cortical circuitry in a dish.

In the mammalian neocortex, local connections as well as projections to and from the cortex are organized in cortical layers. Recent studies have demonstrated that the formation of the patterns of afferent and efferent cortical connections in organotypic co-cultures are similar to those observed in vivo. These findings provide some insights into the cellular strategies that operate during the development of layer-specific cortical connections.