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Literature DB >> 7809075

Genetic selection of growth-inhibitory sequences in mammalian cells.

Abstract

To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.

Entities: Gene Species

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Year: 1994 PMID： 7809075 PMCID： PMC45476 DOI： 10.1073/pnas.91.26.12549

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

51 in total

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3. Construction of a uniform-abundance (normalized) cDNA library.

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Journal: Proc Natl Acad Sci U S A Date: 1991-03-01 Impact factor: 11.205

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Authors: K Kovary; R Bravo
Journal: Mol Cell Biol Date: 1991-09 Impact factor: 4.272

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13 in total

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5. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing.

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