Literature DB >> 7793639

Efficient analysis of cytochrome P4501A catalytic activity, porphyrins, and total proteins in chicken embryo hepatocyte cultures with a fluorescence plate reader.

S W Kennedy1, S P Jones, L J Bastien.   

Abstract

An efficient method for measuring polychlorinated biphenyl-mediated induction of cytochrome P4501A, using the ethoxyresorufin-O-deethylase (EROD) assay, and total porphyrins in chicken embryo hepatocyte cultures is described. Hepatocytes were cultured in 48-well plates, assays were carried out within the wells, and concentrations of the product of the EROD reaction (resorufin), porphyrins, and total proteins were measured in the same wells with a fluorescence plate reader. The method differs from previous methods developed in this laboratory in that three fluorophors were measured in the same well rather than two.

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Year:  1995        PMID： 7793639     DOI： 10.1006/abio.1995.1237

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Ethoxyresorufin-O-deethylase (EROD) induction by TCDD, PeCDF and PCB 126 in bobwhite quail hepatocytes.

Authors:  Stephanie P Jones; Reza Farmahin; Sean W Kennedy
Journal:  Ecotoxicology       Date:  2014-03-12       Impact factor: 2.823

2.  Cytochrome p4501a induced differentially in endothelial cells cultured from different organs of Anguilla rostrata.

Authors:  Rita Anne Garrick; Bruce R Woodin; John J Stegeman
Journal:  In Vitro Cell Dev Biol Anim       Date:  2005 Jan-Feb       Impact factor: 2.416

3.  Fate of Ah receptor agonists during biological treatment of an industrial sludge containing explosives and pharmaceutical residues.

Authors:  Lillemor K Gustavsson; Nina Klee; Helena Olsman; Henner Hollert; Magnus Engwall
Journal:  Environ Sci Pollut Res Int       Date:  2004       Impact factor: 4.223

4.  Synergistic effect of 2,2',4,4',5,5'-hexachlorobiphenyl and 2,3,7,8-tetrachlorodibenzo-p-dioxin on hepatic porphyrin levels in the rat.

Authors:  A P van Birgelen; K M Fase; J van der Kolk; H Poiger; A Brouwer; W Seinen; M van den Berg
Journal:  Environ Health Perspect       Date:  1996-05       Impact factor: 9.031

5.  Hepatic EROD activity is not a useful biomarker of polychlorinated biphenyl exposure in the adult herring gull (Larus argentatus).

Authors:  Sean W Kennedy; Glen A Fox; Stephanie P Jones; Suzanne F Trudeau
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

6.  Sensitivity of bald eagle (Haliaeetus leucocephalus) hepatocyte cultures to induction of cytochrome P4501A by 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Authors:  Sean W Kennedy; Stephanie P Jones; John E Elliott
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

7.  Gonadal feminization and halogenated environmental contaminants in common terns (Sterna hirundo): evidence that ovotestes in male embryos do not persist to the prefledgling stage.

Authors:  Constance A Hart; Ian C T Nisbet; Sean W Kennedy; Mark E Hahn
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

8.  Regulation of cytochrome P4501A1 expression by hyperoxia in human lung cell lines: Implications for hyperoxic lung injury.

Authors:  Kushal Y Bhakta; Weiwu Jiang; Xanthi I Couroucli; Inayat S Fazili; Kathirvel Muthiah; Bhagavatula Moorthy
Journal:  Toxicol Appl Pharmacol       Date:  2008-09-11       Impact factor: 4.219

9.  Characterization of the human hepatocellular carcinoma (hepg2) cell line as an in vitro model for cadmium toxicity studies.

Authors:  P F Dehn; C M White; D E Conners; G Shipkey; T A Cumbo
Journal:  In Vitro Cell Dev Biol Anim       Date:  2004 May-Jun       Impact factor: 2.416

10.  Cytochrome P4501A induction in primary cultures of embryonic European starling hepatocytes exposed to TCDD, PeCDF and TCDF.

Authors:  Reza Farmahin; Doug Crump; Stephanie P Jones; Lukas J Mundy; Sean W Kennedy
Journal:  Ecotoxicology       Date:  2013-03-22       Impact factor: 2.823
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