| Literature DB >> 7790444 |
Abstract
A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.Entities:
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Year: 1995 PMID: 7790444 PMCID: PMC228048 DOI: 10.1128/jcm.33.4.821-823.1995
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948