Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers.

Literature DB >> 7787066

A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers.

M A Ferenczi¹, Z H He, R K Chillingworth, M Brune, J E Corrie, D R Trentham, M R Webb.

Abstract

A new method for the measurement of phosphate release in contracting and relaxed permeabilized muscle fibers is described. The assay is based on a genetically engineered phosphate-binding protein labeled with a coumarin fluorescent probe, which binds inorganic phosphate tightly and shows a fourfold increase in fluorescence upon binding. Measurements of Pi release on the millisecond time scale with sensitivity in the 10 microM range are obtained that provide new information about the relationship between ATP hydrolysis and force production.

Entities: Chemical

Mesh：

Substances：

Year: 1995 PMID： 7787066 PMCID： PMC1281913

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

2 in total

1. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

Authors: M Brune; J L Hunter; J E Corrie; M R Webb
Journal: Biochemistry Date: 1994-07-12 Impact factor: 3.162

2. Myofibrillar ATPase activity and mechanical performance of skinned fibres from rabbit psoas muscle.

Authors: E J Potma; G J Stienen; J P Barends; G Elzinga
Journal: J Physiol Date: 1994-01-15 Impact factor: 5.182

2 in total

8 in total

A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers.

1. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

2. Myofibrillar ATPase activity and mechanical performance of skinned fibres from rabbit psoas muscle.

Review 1. Why choose myofibrils to study muscle myosin ATPase?

2. Real-time measurement of pyrophosphate release kinetics.

3. Rate of phosphate release after photoliberation of adenosine 5'-triphosphate in slow and fast skeletal muscle fibers.

4. ATPase kinetics on activation of rabbit and frog permeabilized isometric muscle fibres: a real time phosphate assay.

5. ATPase activity of myosin in hair bundles of the bullfrog's sacculus.

Review 6. A century of exercise physiology: key concepts in muscle energetics.

7. Time-resolved measurements of phosphate release by cycling cross-bridges in portal vein smooth muscle.

8. Special Issue: The Actin-Myosin Interaction in Muscle: Background and Overview.