Literature DB >> 7772418

A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization.

F J Asturias1, R D Kornberg.   

Abstract

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7772418     DOI： 10.1006/jsbi.1995.1005

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  6 in total

1.  7A projection map of the S-layer protein sbpA obtained with trehalose-embedded monolayer crystals.

Authors:  Julie E Norville; Deborah F Kelly; Thomas F Knight; Angela M Belcher; Thomas Walz
Journal:  J Struct Biol       Date:  2007-06-15       Impact factor: 2.867

2.  Structure of wild-type yeast RNA polymerase II and location of Rpb4 and Rpb7.

Authors:  G J Jensen; G Meredith; D A Bushnell; R D Kornberg
Journal:  EMBO J       Date:  1998-04-15       Impact factor: 11.598

3.  Structure of DNA-dependent protein kinase: implications for its regulation by DNA.

Authors:  K K Leuther; O Hammarsten; R D Kornberg; G Chu
Journal:  EMBO J       Date:  1999-03-01       Impact factor: 11.598

4.  On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy.

Authors:  Dianne W Taylor; Deborah F Kelly; Anchi Cheng; Kenneth A Taylor
Journal:  J Struct Biol       Date:  2007-05-06       Impact factor: 2.867

5.  Lipid monolayer and sparse matrix screening for growing two-dimensional crystals for electron crystallography: methods and examples.

Authors:  Mark Yeager; Kelly A Dryden; Barbie K Ganser-Pornillos
Journal:  Methods Mol Biol       Date:  2013

Review 6.  The use of trehalose in the preparation of specimens for molecular electron microscopy.

Authors:  Po-Lin Chiu; Deborah F Kelly; Thomas Walz
Journal:  Micron       Date:  2011-06-25       Impact factor: 2.251
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.