Literature DB >> 7768445

Linkage map of the honey bee, Apis mellifera, based on RAPD markers.

G J Hunt1, R E Page.   

Abstract

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be approximately 3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

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Year:  1995        PMID： 7768445      PMCID： PMC1206463     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  31 in total

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Journal:  Genomics       Date:  1992-11       Impact factor: 5.736

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Journal:  Genetics       Date:  1992-11       Impact factor: 4.562

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Authors:  L Zheng; F H Collins; V Kumar; F C Kafatos
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  65 in total

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Journal:  Genetics       Date:  1999-12       Impact factor: 4.562

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Authors:  L M Larraya; G Pérez; E Ritter; A G Pisabarro; L Ramírez
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8.  Exceptionally high levels of recombination across the honey bee genome.

Authors:  Martin Beye; Irene Gattermeier; Martin Hasselmann; Tanja Gempe; Morten Schioett; John F Baines; David Schlipalius; Florence Mougel; Christine Emore; Olav Rueppell; Anu Sirviö; Ernesto Guzmán-Novoa; Greg Hunt; Michel Solignac; Robert E Page
Journal:  Genome Res       Date:  2006-10-25       Impact factor: 9.043

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Authors:  Florence Mougel; Marie-Anne Poursat; Nicolas Beaume; Dominique Vautrin; Michel Solignac
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10.  Genomic analysis in the sting-2 quantitative trait locus for defensive behavior in the honey bee, Apis mellifera.

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