Literature DB >> 7767897

Formation and repair of 1,3-bis-(2-chloroethyl)-1-nitrosourea and cisplatin induced total genomic DNA interstrand crosslinks in human glioma cells.

F Ali-Osman1, A Rairkar, P Young.   

Abstract

The kinetics of formation and repair of total genomic DNA interstrand crosslinks (ISCs) induced by BCNU and cis-DDP were studied in cells of 6 human malignant gliomas and related with their degree of drug resistance. DNA ISCs were formed rapidly (peak 6-12 h) following a 2 h exposure to 50 microM BCNU or 25 uM cis-DDP, and on an equimolar basis higher levels of crosslinking were observed with cis-DDP than with BCNU. Repair of cis-DDP induced crosslinks was characteristically bi-phasic and the rate was significantly higher than that for BCNU induced crosslinks. Overall, a low crosslink index and a high crosslink repair rate correlated with cis-DDP and BCNU resistance. The data demonstrate, conclusively, the ability of human glioma cells to repair cis-DDP and, for the first time, BCNU induced DNA ISCs and that DNA crosslink repair is a significant contributing factor to the resistance of these tumors to the two agents.

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Year:  1995        PMID： 7767897

Source DB:  PubMed          Journal:  Cancer Biochem Biophys        ISSN： 0305-7232


  7 in total

1.  Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro.

Authors:  S K Chintala; F Ali-Osman; S Mohanam; A Rayford; Y Go; Z L Gokaslan; E Gagercas; B Venkaiah; R Sawaya; G L Nicolson; J S Rao
Journal:  Clin Exp Metastasis       Date:  1997-07       Impact factor: 5.150

2.  Rad5 coordinates translesion DNA synthesis pathway by recognizing specific DNA structures in saccharomyces cerevisiae.

Authors:  Qifu Fan; Xin Xu; Xi Zhao; Qian Wang; Wei Xiao; Ying Guo; Yu V Fu
Journal:  Curr Genet       Date:  2018-02-02       Impact factor: 3.886

3.  Correlation of clinical features and methylation status of MGMT gene promoter in glioblastomas.

Authors:  J L Blanc; M Wager; J Guilhot; S Kusy; B Bataille; T Chantereau; F Lapierre; C J Larsen; L Karayan-Tapon
Journal:  J Neurooncol       Date:  2004-07       Impact factor: 4.130

4.  Disulfiram is a direct and potent inhibitor of human O6-methylguanine-DNA methyltransferase (MGMT) in brain tumor cells and mouse brain and markedly increases the alkylating DNA damage.

Authors:  Ameya Paranjpe; Ruiwen Zhang; Francis Ali-Osman; George C Bobustuc; Kalkunte S Srivenugopal
Journal:  Carcinogenesis       Date:  2013-11-05       Impact factor: 4.944

5.  New insights into estrogenic regulation of O6-methylguanine DNA-methyltransferase (MGMT) in human breast cancer cells: Co-degradation of ER-α and MGMT proteins by fulvestrant or O6-benzylguanine indicates fresh avenues for therapy.

Authors:  Ameya Paranjpe; Nathan I Bailey; Santhi Konduri; George C Bobustuc; Francis Ali-Osman; Mohd A Yusuf; Surendra R Punganuru; Hanumantha Rao Madala; Debasish Basak; Agm Mostofa; Kalkunte S Srivenugopal
Journal:  J Biomed Res       Date:  2016-06-10

6.  The SOS and RpoS Regulons Contribute to Bacterial Cell Robustness to Genotoxic Stress by Synergistically Regulating DNA Polymerase Pol II.

Authors:  Tanja Dapa; Sébastien Fleurier; Marie-Florence Bredeche; Ivan Matic
Journal:  Genetics       Date:  2017-05-03       Impact factor: 4.562

7.  S-phase Specific Downregulation of Human O6-Methylguanine DNA Methyltransferase (MGMT) and its Serendipitous Interactions with PCNA and p21cip1 Proteins in Glioma Cells.

Authors:  Agm Mostofa; Surendra R Punganuru; Hanumantha Rao Madala; Kalkunte S Srivenugopal
Journal:  Neoplasia       Date:  2018-03-03       Impact factor: 5.715
  7 in total

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