Literature DB >> 7766136

The resistance of cellulases and xylanases to proteolytic inactivation.

C M Fontes1, J Hall, B H Hirst, G P Hazlewood, H J Gilbert.   

Abstract

The sensitivity of a range of cellulases and xylanases to proteolytic inactivation was investigated. The xylanases, all the Clostridium thermocellum cellulases and cellulase E from Pseudomonas fluorescens subsp. cellulosa exhibited no decrease in catalytic activity during a 3-h incubation with proteinases of the small intestine. Under these conditions, the control Escherichia coli enzymes analysed had half-lives of 4.3-13.5 min. The addition of substrate significantly decreased the sensitivity of proteinase-labile enzymes to inactivation. The significance of these data in relation to the use of cellulases and xylanases for improving animal nutrition is discussed.

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Year:  1995        PMID： 7766136     DOI： 10.1007/BF00170622

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  17 in total

Review 1.  Genetic modification of fibre digestion.

Authors:  H J Gilbert; G P Hazlewood
Journal:  Proc Nutr Soc       Date:  1991-08       Impact factor: 6.297

2.  Cloning and sequencing of the celA gene encoding endoglucanase A of Butyrivibrio fibrisolvens strain A46.

Authors:  G P Hazlewood; K Davidson; J I Laurie; M P Romaniec; H J Gilbert
Journal:  J Gen Microbiol       Date:  1990-10

3.  Multiple xylanases of Cellulomonas fimi are encoded by distinct genes.

Authors:  J H Clarke; J I Laurie; H J Gilbert; G P Hazlewood
Journal:  FEMS Microbiol Lett       Date:  1991-10-15       Impact factor: 2.742

Review 4.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

5.  Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.

Authors:  P Béguin; P Cornet; J P Aubert
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

6.  Identification of regulatory elements of cloned genes with functional assays.

Authors:  N Rosenthal
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

7.  Manipulation of the repertoire of digestive enzymes secreted into the gastrointestinal tract of transgenic mice.

Authors:  J Hall; S Ali; M A Surani; G P Hazlewood; A J Clark; J P Simons; B H Hirst; H J Gilbert
Journal:  Biotechnology (N Y)       Date:  1993-03

8.  Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes.

Authors:  L E Kellett; D M Poole; L M Ferreira; A J Durrant; G P Hazlewood; H J Gilbert
Journal:  Biochem J       Date:  1990-12-01       Impact factor: 3.857

9.  Gene sequence and properties of CelI, a family E endoglucanase from Clostridium thermocellum.

Authors:  G P Hazlewood; K Davidson; J I Laurie; N S Huskisson; H J Gilbert
Journal:  J Gen Microbiol       Date:  1993-02

10.  Endoglucanase E, produced at high level in Escherichia coli as a lacZ' fusion protein, is part of the Clostridium thermocellum cellulosome.

Authors:  G P Hazlewood; K Davidson; J H Clarke; A J Durrant; J Hall; H J Gilbert
Journal:  Enzyme Microb Technol       Date:  1990-09       Impact factor: 3.493
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  13 in total

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2.  Properties of an alkali-thermo stable xylanase from Geobacillus thermodenitrificans A333 and applicability in xylooligosaccharides generation.

Authors:  Loredana Marcolongo; Francesco La Cara; Alessandra Morana; Anna Di Salle; Giovanni Del Monaco; Susana M Paixão; Luis Alves; Elena Ionata
Journal:  World J Microbiol Biotechnol       Date:  2015-02-17       Impact factor: 3.312

3.  Purification and characterization of two different xylanases from the thermophilic actinomycete Microtetraspora flexuosa SIIX.

Authors:  S Berens; H Kaspari; J H Klemme
Journal:  Antonie Van Leeuwenhoek       Date:  1996-04       Impact factor: 2.271

4.  Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase.

Authors:  M H Beylot; K Emami; V A McKie; H J Gilbert; G Pell
Journal:  Biochem J       Date:  2001-09-15       Impact factor: 3.857

5.  The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan.

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Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

6.  Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose.

Authors:  Shabir Najmudin; Benedita A Pinheiro; Maria J Romão; José A M Prates; Carlos M G A Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-07-05

Review 7.  Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus.

Authors:  Jeffrey G Gardner
Journal:  World J Microbiol Biotechnol       Date:  2016-06-04       Impact factor: 3.312

8.  The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel.

Authors:  J Hall; G W Black; L M Ferreira; S J Millward-Sadler; B R Ali; G P Hazlewood; H J Gilbert
Journal:  Biochem J       Date:  1995-08-01       Impact factor: 3.857

9.  Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

Authors:  Soumyadeep Chakraborty; Vania O Fernandes; Fernando M V Dias; Jose A M Prates; Luis M A Ferreira; Carlos M G A Fontes; Arun Goyal; Maria S J Centeno
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10.  Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall.

Authors:  Diogo Coelho; Paula A Lopes; Vânia Cardoso; Patrícia Ponte; Joana Brás; Marta S Madeira; Cristina M Alfaia; Narcisa M Bandarra; Henri G Gerken; Carlos M G A Fontes; José A M Prates
Journal:  Sci Rep       Date:  2019-03-29       Impact factor: 4.379
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