Literature DB >> 7765933

Effect of lipid supplements on the production and glycosylation of recombinant interferon-gamma expressed in CHO cells.

N Jenkins1, P Castro, S Menon, A Ison, A Bull.   

Abstract

The effects of lipids on the glycosylation of recombinant human interferon-gamma expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-gamma deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-gamma glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7765933     DOI： 10.1007/BF00762395

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  25 in total

Review 1.  Glycosylation of recombinant protein therapeutics: control and functional implications.

Authors:  D A Cumming
Journal:  Glycobiology       Date:  1991-03       Impact factor: 4.313

2.  Production of tPA in recombinant CHO cells under oxygen-limited conditions.

Authors:  A A Lin; R Kimura; W M Miller
Journal:  Biotechnol Bioeng       Date:  1993-07       Impact factor: 4.530

Review 3.  Biosynthesis of N-acetylglucosamine-P-P-dolichol, the committed step of asparagine-linked oligosaccharide assembly.

Authors:  M A Lehrman
Journal:  Glycobiology       Date:  1991-12       Impact factor: 4.313

4.  1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon-gamma.

Authors:  S Grzesiek; H Döbeli; R Gentz; G Garotta; A M Labhardt; A Bax
Journal:  Biochemistry       Date:  1992-09-08       Impact factor: 3.162

5.  Cell-free synthesis of enzymically active tissue-type plasminogen activator. Protein folding determines the extent of N-linked glycosylation.

Authors:  N J Bulleid; R S Bassel-Duby; R B Freedman; J F Sambrook; M J Gething
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

6.  Three-dimensional structure of recombinant human interferon-gamma.

Authors:  S E Ealick; W J Cook; S Vijay-Kumar; M Carson; T L Nagabhushan; P P Trotta; C E Bugg
Journal:  Science       Date:  1991-05-03       Impact factor: 47.728

Review 7.  The molecular cell biology of interferon-gamma and its receptor.

Authors:  M A Farrar; R D Schreiber
Journal:  Annu Rev Immunol       Date:  1993       Impact factor: 28.527

8.  Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture.

Authors:  P M Hayter; E M Curling; A J Baines; N Jenkins; I Salmon; P G Strange; A T Bull
Journal:  Appl Microbiol Biotechnol       Date:  1991-02       Impact factor: 4.813

Review 9.  The oligosaccharides of glycoproteins: bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties.

Authors:  C F Goochee; M J Gramer; D C Andersen; J B Bahr; J R Rasmussen
Journal:  Biotechnology (N Y)       Date:  1991-12

10.  High-resolution separation of recombinant human interferon-gamma glycoforms by micellar electrokinetic capillary chromatography.

Authors:  D C James; R B Freedman; M Hoare; N Jenkins
Journal:  Anal Biochem       Date:  1994-11-01       Impact factor: 3.365
View more
  13 in total

1.  Some myths and messages concerning the batch and continuous culture of animal cells.

Authors:  A Kadouri; R E Spier
Journal:  Cytotechnology       Date:  1997-07       Impact factor: 2.058

2.  Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells and its effects on the sialylation of recombinant interferon-γ.

Authors:  L Monaco; A Marc; A Eon-Duval; G Acerbis; G Distefano; D Lamotte; J M Engasser; M Soria; N Jenkins
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

3.  On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.

Authors:  Y H Guan; R B Kemp
Journal:  Cytotechnology       Date:  1999-07       Impact factor: 2.058

4.  Related effects of cell adaptation to serum-free conditions on murine EPO production and glycosylation by CHO cells.

Authors:  François Lefloch; Bertrand Tessier; Sébastien Chenuet; Jean-Marc Guillaume; Pierre Cans; Jean-Louis Goergen; Annie Marc
Journal:  Cytotechnology       Date:  2006-12-29       Impact factor: 2.058

5.  Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation.

Authors:  Jintao Liu; Jie Wang; Li Fan; Xinning Chen; Dongdong Hu; Xiancun Deng; H Fai Poon; Haibin Wang; Xuping Liu; Wen-Song Tan
Journal:  World J Microbiol Biotechnol       Date:  2015-05-01       Impact factor: 3.312

6.  The effect of nitrogen source on yield and glycosylation of a human cystatin C mutant expressed in Pichia pastoris.

Authors:  Jason Pritchett; Susan A Baldwin
Journal:  J Ind Microbiol Biotechnol       Date:  2004-11-18       Impact factor: 3.346

7.  CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids.

Authors:  P M Castro; A P Ison; P M Hayter; A T Bull
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

8.  Na-butyrate increases the production and alpha2,6-sialylation of recombinant interferon-gamma expressed by alpha2,6- sialyltransferase engineered CHO cells.

Authors:  D Lamotte; L Buckberry; L Monaco; M Soria; N Jenkins; J M Engasser; A Marc
Journal:  Cytotechnology       Date:  1999-01       Impact factor: 2.058

9.  Enhanced erythropoietin heterogeneity in a CHO culture is caused by proteolytic degradation and can be eliminated by a high glutamine level.

Authors:  M Yang; M Butler
Journal:  Cytotechnology       Date:  2000-10       Impact factor: 2.058

10.  Biological Insights into Therapeutic Protein Modifications throughout Trafficking and Their Biopharmaceutical Applications.

Authors:  Xiaotian Zhong; Jill F Wright
Journal:  Int J Cell Biol       Date:  2013-04-18
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.